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Western Blot (WB) ((0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat BLK Polyclonal Antibody | anti-BLK antibody

Goat anti-BLK Antibody

Gene Names
BLK; MODY11
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BLK; Polyclonal Antibody; Goat anti-BLK Antibody; B lymphoid tyrosine kinase; MGC10442; MODY11; BLK nonreceptor tyrosine kinase; b lymphocyte kinase; p55-Blk; tyrosine-protein kinase Blk; BLK antibody; B lymphoid tyrosine kinase antibody; MGC10442 antibody; MODY11 antibody; antibody; anti-BLK antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QWSPLKVSAQDKD
Sequence Length
505
Applicable Applications for anti-BLK antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 50kDa band observed in Human Thymus, Spleen and Lymph Node lysates (calculated MW of 57.7kDa according to NP_001706.2). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence QWSPLKVSAQDKD-C, from the N Terminus (near) of the protein sequence according to NP_001706.2.
Epitope
N Terminus (near)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
640
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,706 Da
NCBI Official Full Name
tyrosine-protein kinase Blk
NCBI Official Synonym Full Names
BLK proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
BLK
NCBI Official Synonym Symbols
MODY11
NCBI Protein Information
tyrosine-protein kinase Blk
UniProt Protein Name
Tyrosine-protein kinase Blk
Protein Family
UniProt Gene Name
BLK
UniProt Entry Name
BLK_HUMAN

NCBI Description

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

Uniprot Description

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: B cell receptor signaling pathway; cell differentiation; cytoskeleton organization and biogenesis; innate immune response; negative regulation of apoptosis; positive regulation of insulin secretion; regulation of cell proliferation; regulation of phagocytosis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Maturity-onset Diabetes Of The Young, Type 11

Research Articles on BLK

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Product Notes

The BLK blk (Catalog #AAA422608) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BLK Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's BLK can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 50kDa band observed in Human Thymus, Spleen and Lymph Node lysates (calculated MW of 57.7kDa according to NP_001706.2). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the BLK blk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QWSPLKVSAQ DKD. It is sometimes possible for the material contained within the vial of "BLK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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