Western Blot (WB)
((0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TRIM71 Polyclonal Antibody | anti-TRIM71 antibody
Goat anti-TRIM71 (aa390-402) Antibody
Gene Names
TRIM71; LIN41; LIN-41
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TRIM71; Polyclonal Antibody; Goat anti-TRIM71 (aa390-402) Antibody; Goat Anti-TRIM71 (aa409-419) Antibody; tripartite motif-containing 71; LIN-41; LIN41; abnormal cell LINeage LIN-41; homolog of C. elegans Lin-41; abnormal cell LINeage LIN-41 antibody; homolog of C. elegans Lin-41 antibody; LIN41 antibody; LIN-41 antibody; tripartite motif-containing 71 antibody; TRIM71 antibody; TRIM71 (aa390-402); anti-TRIM71 antibody
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RQVKAKSLYLQVE
Sequence Length
868
Applicable Applications for anti-TRIM71 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 100kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4kDa according to NP_001034200.1). Recommended concentration: 0.5-1ug/ml.
Western Blot: Approx 100kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4kDa according to NP_001034200.1). Recommended concentration: 0.5-1ug/ml.
Immunogen
Peptide with sequence C-RQVKAKSLYLQVE, from the internal region of the protein sequence according to NP_001034200.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,385 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM71
NCBI Official Synonym Full Names
tripartite motif containing 71, E3 ubiquitin protein ligase
NCBI Official Symbol
TRIM71
NCBI Official Synonym Symbols
LIN41; LIN-41
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM71
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM71
Protein Family
UniProt Gene Name
TRIM71
UniProt Synonym Gene Names
LIN41
UniProt Entry Name
LIN41_HUMAN
NCBI Description
The protein encoded by this gene is an E3 ubiquitin-protein ligase that binds with miRNAs and maintains the growth and upkeep of embryonic stem cells. This gene also is involved in the G1-S phase transition of the cell cycle. [provided by RefSeq, Dec 2015]
Uniprot Description
TRIM71: May be involved in controlling the timing of organ formation during development. Belongs to the TRIM/RBCC family.
Chromosomal Location of Human Ortholog: 3p22.3
Molecular Function: ligase activity; miRNA binding; translation repressor activity; ubiquitin-protein ligase activity; zinc ion binding
Biological Process: fibroblast growth factor receptor signaling pathway; G1/S transition of mitotic cell cycle; miRNA-mediated gene silencing, negative regulation of translation; negative regulation of translation; neural tube closure; neural tube development; protein autoubiquitination
Research Articles on TRIM71
Similar Products
Product Notes
The TRIM71 trim71 (Catalog #AAA422326) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TRIM71 (aa390-402) Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM71 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 100kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4kDa according to NP_001034200.1). Recommended concentration: 0.5-1ug/ml. Researchers should empirically determine the suitability of the TRIM71 trim71 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RQVKAKSLYL QVE. It is sometimes possible for the material contained within the vial of "TRIM71, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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