Goat DTX1/Deltex-1 Polyclonal Antibody | anti-DTX1 antibody
Goat anti-DTX1/Deltex-1 Antibody
Western Blot: Preliminary experiments gave an approx 48kDa band in Mouse Fetal Brain lysates and in lysates of NIH3T3 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.4kDa according to Human NP_004407.2 and of 68.2kDa according to Mouse NP_032078.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI and Uniprot Product Information
NCBI Description
Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity. [provided by RefSeq, Jul 2008]
Uniprot Description
DTX1: Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity. Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T- cell development, suggesting that it can antagonize NOTCH1. Belongs to the Deltex family.
Protein type: Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 12q24.13
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: ligase activity; Notch binding; protein binding; SH3 domain binding; transcription coactivator activity; ubiquitin protein ligase binding; zinc ion binding
Biological Process: cell surface receptor linked signal transduction; glial cell differentiation; negative regulation of neuron differentiation; negative regulation of T cell differentiation; Notch signaling pathway; protein ubiquitination; regulation of Notch signaling pathway; transcription from RNA polymerase II promoter; transcription, DNA-dependent
Research Articles on DTX1
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Product Notes
The DTX1 dtx1 (Catalog #AAA422126) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DTX1/Deltex-1 Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's DTX1/Deltex-1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Preliminary experiments gave an approx 48kDa band in Mouse Fetal Brain lysates and in lysates of NIH3T3 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.4kDa according to Human NP_004407.2 and of 68.2kDa according to Mouse NP_032078.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the DTX1 dtx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CHHIENVLKE DAR. It is sometimes possible for the material contained within the vial of "DTX1/Deltex-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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