Western Blot (WB)
((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat HIP14L/ZDHHC13 Polyclonal Antibody | anti-ZDHHC13 antibody
Goat anti-HIP14L/ZDHHC13 Antibody
Gene Names
ZDHHC13; HIP14L; HIP3RP
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
HIP14L/ZDHHC13; Polyclonal Antibody; Goat anti-HIP14L/ZDHHC13 Antibody; ZDHHC13; HIP14L; HIP3RP; FLJ10852; FLJ10941; MGC64994; zinc finger; DHHC domain containing 13; HIP14-like; HIP14-related protein; huntingtin interacting protein HIP3RP; hypothetical protein similar to ankyrin repeat-containing priotein AKR1; ; ZDHHC13 antibody; HIP14L antibody; HIP3RP antibody; FLJ10852 antibody; FLJ10941 antibody; MGC64994 antibody; DHHC domain containing 13 antibody; HIP14-like antibody; HIP14-related protein antibody; huntingtin interacting protein HIP3RP antibody; hypothetical protein similar to ankyrin repeat-containing priotein AKR1 antibody; DHHC-type containing 13 antibody; HIP14L / ZDHHC13; anti-ZDHHC13 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
FHPAREKVLRSV
Sequence Length
622
Applicable Applications for anti-ZDHHC13 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx.70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 70.9kDa according to NP_061901.2). Recommended concentration: 1-3ug/ml.
Western Blot: Approx.70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 70.9kDa according to NP_061901.2). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-FHPAREKVLRSV, from the C Terminus of the protein sequence according to NP_061901.2; NP_001001483.1.
Epitope
C Terminus
Note
This antibody is expected to recognise both reported isoforms (NP_061901.2 and NP_001001483.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,509 Da
NCBI Official Full Name
palmitoyltransferase ZDHHC13 isoform 1
NCBI Official Synonym Full Names
zinc finger DHHC-type containing 13
NCBI Official Symbol
ZDHHC13
NCBI Official Synonym Symbols
HIP14L; HIP3RP
NCBI Protein Information
palmitoyltransferase ZDHHC13
UniProt Protein Name
Palmitoyltransferase ZDHHC13
Protein Family
UniProt Gene Name
ZDHHC13
UniProt Synonym Gene Names
DHHC-13
UniProt Entry Name
ZDH13_HUMAN
Uniprot Description
ZDHHC13: Palmitoyltransferase for HD and GAD2. Mediates Mg(2+) transport. Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.3.1.225; Membrane protein, multi-pass; Transferase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: endoplasmic reticulum; Golgi apparatus; Golgi membrane; integral to membrane; intracellular membrane-bound organelle; membrane
Molecular Function: magnesium ion transmembrane transporter activity; palmitoyltransferase activity; protein-cysteine S-palmitoleyltransferase activity; signal transducer activity; zinc ion binding
Biological Process: metabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction
Research Articles on ZDHHC13
Similar Products
Product Notes
The ZDHHC13 zdhhc13 (Catalog #AAA421991) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HIP14L/ZDHHC13 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HIP14L/ZDHHC13 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx.70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 70.9kDa according to NP_061901.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ZDHHC13 zdhhc13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FHPAREKVLR SV. It is sometimes possible for the material contained within the vial of "HIP14L/ZDHHC13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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