Goat DUBA2 Polyclonal Antibody | anti-OTUD6A antibody
Goat anti-DUBA2 Antibody
Gene Names
OTUD6A; DUBA2; HSHIN6
Reactivity
Expected from sequence similarity: Human
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
DUBA2; Polyclonal Antibody; Goat anti-DUBA2 Antibody; OTUD6A; OTU domain containing 6A; FLJ25831; HSHIN6; HIN-6 protease; OTUD6A antibody; OTU domain containing 6A antibody; DUBA2 antibody; FLJ25831 antibody; HSHIN6 antibody; HIN-6 protease antibody; anti-OTUD6A antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RCRTASYMKKHVDE
Sequence Length
288
Applicable Applications for anti-OTUD6A antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Preliminary experiments gave an approx 22-23kDa band in Human Pancreas, Human Ovary and Human Kidney lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.3kDa according to NP_997203.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 22-23kDa band in Human Pancreas, Human Ovary and Human Kidney lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.3kDa according to NP_997203.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence RCRTASYMKKHVDE, from the internal region of the protein sequence according to NP_997203.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,300 Da
NCBI Official Full Name
OTU domain-containing protein 6A
NCBI Official Synonym Full Names
OTU deubiquitinase 6A
NCBI Official Symbol
OTUD6A
NCBI Official Synonym Symbols
DUBA2; HSHIN6
NCBI Protein Information
OTU domain-containing protein 6A
UniProt Protein Name
OTU domain-containing protein 6A
Protein Family
UniProt Gene Name
OTUD6A
UniProt Synonym Gene Names
DUBA2
UniProt Entry Name
OTU6A_HUMAN
NCBI Description
Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA2 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM, May 2008]
Uniprot Description
OTUD6A: Deubiquitinating enzyme that hydrolyzes 'Lys-27'-, 'Lys- 29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains
Protein type: Ubiquitin-specific protease; EC 3.4.19.12
Chromosomal Location of Human Ortholog: Xq13.1
Molecular Function: ubiquitin-specific protease activity
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Product Notes
The OTUD6A otud6a (Catalog #AAA421922) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DUBA2 Antibody reacts with Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's DUBA2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Preliminary experiments gave an approx 22-23kDa band in Human Pancreas, Human Ovary and Human Kidney lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.3kDa according to NP_997203.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the OTUD6A otud6a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RCRTASYMKK HVDE. It is sometimes possible for the material contained within the vial of "DUBA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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