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Western Blot (WB) ((0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat APOA1BP Polyclonal Antibody | anti-APOA1BP antibody

Goat anti-APOA1BP Antibody

Gene Names
NAXE; AIBP; YJEFN1; APOA1BP
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
APOA1BP; Polyclonal Antibody; Goat anti-APOA1BP Antibody; apolipoprotein A-I binding protein; AIBP; MGC119143; MGC119144; MGC119145; apoA-I binding protein; APOA1BP antibody; apolipoprotein A-I binding protein antibody; AIBP antibody; MGC119143 antibody; MGC119144 antibody; MGC119145 antibody; apoA-I binding protein antibody; anti-APOA1BP antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PPYPDTECVYRLQ
Sequence Length
288
Applicable Applications for anti-APOA1BP antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 29kDa band observed in Human Heart lysates (calculated MW of 31.7kDa according to NP_658985.2). Recommended concentration: 0.5-1.5ug/ml.
Immunogen
Peptide with sequence PPYPDTECVYRLQ, from the C Terminus of the protein sequence according to NP_658985.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,431 Da
NCBI Official Full Name
NAD(P)H-hydrate epimerase
NCBI Official Synonym Full Names
NAD(P)HX epimerase
NCBI Official Symbol
NAXE
NCBI Official Synonym Symbols
AIBP; YJEFN1; APOA1BP
NCBI Protein Information
NAD(P)H-hydrate epimerase
UniProt Protein Name
NAD(P)H-hydrate epimerase
UniProt Gene Name
APOA1BP
UniProt Synonym Gene Names
AI-BP; YjeF_N1
UniProt Entry Name
NNRE_HUMAN

NCBI Description

The product of this gene interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I. The human genome contains related pseudogenes. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA1BP: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S- specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. Belongs to the nnrE/AIBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 5.1.99.6

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cilium; cytoplasm; cytosol; extracellular region; extracellular space; intracellular membrane-bound organelle; mitochondrial matrix; mitochondrion; nucleoplasm

Molecular Function: metal ion binding; nucleotide binding; protein binding; protein homodimerization activity

Biological Process: nicotinamide metabolic process; protein homotetramerization

Research Articles on APOA1BP

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Product Notes

The APOA1BP apoa1bp (Catalog #AAA421813) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-APOA1BP Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's APOA1BP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 29kDa band observed in Human Heart lysates (calculated MW of 31.7kDa according to NP_658985.2). Recommended concentration: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the APOA1BP apoa1bp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PPYPDTECVY RLQ. It is sometimes possible for the material contained within the vial of "APOA1BP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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