Goat LAX Polyclonal Antibody | anti-LAX1 antibody
Goat anti-LAX Antibody
Gene Names
LAX1; LAX
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
LAX; Polyclonal Antibody; Goat anti-LAX Antibody; Goat Anti-LAX1 Antibody; LAX1; FLJ20340; LAT-like membrane associated protein; lymphocyte transmembrane adaptor 1; linker for activation of x cells; LAX1 antibody; LAX antibody; FLJ20340 antibody; LAT-like membrane associated protein antibody; lymphocyte transmembrane adaptor 1 antibody; linker for activation of x cells antibody; anti-LAX1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PLLTLPQTRQRA
Sequence Length
398
Applicable Applications for anti-LAX1 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave bands at approx.100kDa and 26kDa in Human Tonsil lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 44.1kDa according to NP_060243.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Western Blot: Preliminary experiments gave bands at approx.100kDa and 26kDa in Human Tonsil lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 44.1kDa according to NP_060243.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunogen
Peptide with sequence C-PLLTLPQTRQRA, from the internal region of the protein sequence according to NP_060243.2; NP_001129662.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,491 Da
NCBI Official Full Name
lymphocyte transmembrane adapter 1 isoform a
NCBI Official Synonym Full Names
lymphocyte transmembrane adaptor 1
NCBI Official Symbol
LAX1
NCBI Official Synonym Symbols
LAX
NCBI Protein Information
lymphocyte transmembrane adapter 1
UniProt Protein Name
Lymphocyte transmembrane adapter 1
Protein Family
UniProt Gene Name
LAX1
UniProt Synonym Gene Names
LAX
UniProt Entry Name
LAX1_HUMAN
Uniprot Description
LAX1: Negatively regulates TCR (T-cell antigen receptor)- mediated signaling in T-cells and BCR (B-cell antigen receptor)- mediated signaling in B-cells. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: cytoplasm; Golgi apparatus; integral to membrane; lipid raft; membrane; plasma membrane
Molecular Function: protein binding; protein kinase binding; SH2 domain binding
Biological Process: adaptive immune response; antigen receptor-mediated signaling pathway; B cell activation; immune response; immunoglobulin secretion; inactivation of MAPK activity; negative regulation of T cell activation
Research Articles on LAX1
Similar Products
Product Notes
The LAX1 lax1 (Catalog #AAA421638) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LAX Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAX can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave bands at approx.100kDa and 26kDa in Human Tonsil lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 44.1kDa according to NP_060243.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Researchers should empirically determine the suitability of the LAX1 lax1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PLLTLPQTRQ RA. It is sometimes possible for the material contained within the vial of "LAX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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