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Western Blot (WB) ((0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat RASSF8 Polyclonal Antibody | anti-RASSF8 antibody

Goat anti-RASSF8 Antibody

Gene Names
RASSF8; HOJ1; C12orf2
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RASSF8; Polyclonal Antibody; Goat anti-RASSF8 Antibody; Ras association (RalGDS/AF-6) domain family 8; C12orf2; HoJ-1; carcinoma associated HOJ-1; RASSF8 antibody; Ras association (RalGDS/AF-6) domain family 8 antibody; C12orf2 antibody; HoJ-1 antibody; carcinoma associated HOJ-1 antibody; anti-RASSF8 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RDTERHLAPHEN
Sequence Length
392
Applicable Applications for anti-RASSF8 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 40-45kDa band observed in Human Testis lysates (calculated MW of 45.4kDa according to NP_009142.2). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-RDTERHLAPHEN, from the internal region of the protein sequence according to NP_009142.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,446 Da
NCBI Official Full Name
ras association domain-containing protein 8 isoform b
NCBI Official Synonym Full Names
Ras association domain family member 8
NCBI Official Symbol
RASSF8
NCBI Official Synonym Symbols
HOJ1; C12orf2
NCBI Protein Information
ras association domain-containing protein 8
UniProt Protein Name
Ras association domain-containing protein 8
UniProt Gene Name
RASSF8
UniProt Synonym Gene Names
C12orf2
UniProt Entry Name
RASF8_HUMAN

NCBI Description

This gene encodes a member of the Ras-assocation domain family (RASSF) of tumor suppressor proteins. This gene is essential for maintaining adherens junction function in epithelial cells and has a role in epithelial cell migration. It is a lung tumor suppressor gene candidate. A chromosomal translocation t(12;22)(p11.2;q13.3) leading to the fusion of this gene and the FBLN1 gene is found in a complex type of synpolydactyly. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

RASSF8: A chromosomal aberration involving RASSF8 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 12p12.3

Biological Process: signal transduction

Research Articles on RASSF8

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Product Notes

The RASSF8 rassf8 (Catalog #AAA421615) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RASSF8 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RASSF8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 40-45kDa band observed in Human Testis lysates (calculated MW of 45.4kDa according to NP_009142.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the RASSF8 rassf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RDTERHLAPH EN. It is sometimes possible for the material contained within the vial of "RASSF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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