Western Blot (WB)
((1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Glutathione Peroxidase 1 Polyclonal Antibody | anti-GPX1 antibody
Goat anti-Glutathione Peroxidase 1 (isoform1) Antibody
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration, 3-5ug/ml. Frozen sections of Porcin Kidney shows staining of cytoplasm in PCT epithelial cells. Recommended concentration, 20ug/ml.
Western Blot: Approx 22kDa band observed in Human Liver and Kidney lysates (calculated MW of 22.1kDa according to NP_000572.2). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunofluorescence (IF)
((20ug/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia)
Immunofluorescence (IF)
((20ug/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family.
Protein type: EC 1.11.1.9; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Oxidoreductase
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: cytoplasm; cytosol; mitochondrial matrix; mitochondrion
Molecular Function: glutathione peroxidase activity; SH3 domain binding
Biological Process: angiogenesis involved in wound healing; blood vessel endothelial cell migration; cell redox homeostasis; endothelial cell development; fat cell differentiation; glutathione metabolic process; heart contraction; hydrogen peroxide catabolic process; induction of apoptosis by oxidative stress; interaction with symbiont; lipoxygenase pathway; myoblast proliferation; negative regulation of caspase activity; negative regulation of inflammatory response to antigenic stimulus; positive regulation of protein kinase B signaling cascade; protein amino acid oxidation; purine nucleotide catabolic process; regulation of gene expression, epigenetic; regulation of mammary gland epithelial cell proliferation; regulation of neuron apoptosis; response to gamma radiation; response to hydrogen peroxide; response to hydroperoxide; response to reactive oxygen species; response to selenium ion; response to symbiotic bacterium; response to xenobiotic stimulus; sensory perception of sound; skeletal muscle fiber development; skeletal muscle regeneration; thermoregulation; triacylglycerol metabolic process; UV protection; vasodilation
Disease: Glutathione Peroxidase Deficiency
Research Articles on GPX1
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Product Notes
The GPX1 gpx1 (Catalog #AAA421332) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Glutathione Peroxidase 1 (isoform1) Antibody reacts with Tested: Human, Pig; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione Peroxidase 1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration, 3-5ug/ml. Frozen sections of Porcin Kidney shows staining of cytoplasm in PCT epithelial cells. Recommended concentration, 20ug/ml. Western Blot: Approx 22kDa band observed in Human Liver and Kidney lysates (calculated MW of 22.1kDa according to NP_000572.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the GPX1 gpx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: REALPAPSDD ATA. It is sometimes possible for the material contained within the vial of "Glutathione Peroxidase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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