Mouse anti-Human GABPA Monoclonal Antibody | anti-GABPA antibody

GABPA (GA-binding Protein alpha Chain, GABP Subunit alpha, Nuclear Respiratory Factor 2 Subunit alpha, Transcription Factor E4TF1-60, E4TF1A) (HRP)

Cat. Num. AAA6152610

• Gene Names: GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GABPA; Monoclonal Antibody; GABPA (GA-binding Protein alpha Chain; GABP Subunit alpha; Nuclear Respiratory Factor 2 Subunit alpha; Transcription Factor E4TF1-60; E4TF1A) (HRP); anti-GABPA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4B8-G6
• Specificity: Recognizes human GABPA.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-GABPA antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-454 from human GABPA (AAH35031) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNILEIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITLDGTKHITTISDETSEQVTRWAAALEGYRKEQERLGIPYDPIQWSTDQVLHWVVWVMKEFSMTDIDLTTLNISGRELCSLNQEDFFQRVPRGEILWSHLELLRKYVLASQEQQMNEIVTIDQPVQIIPASVQSATPTTIKVINSSAKAAKVQRAPRISGEDRSSPGNRTGNNGQIQLWQFLLELLTDKDARDCISWVGDEGEFKLNQPELVAQKWGQRKNKPTMNYEKLSRALRYYYDGDMICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVTECEQKKLAKMQLHGIAQPVTAVALSTASLQTEKDN
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (75.68kD).)

Western Blot (WB)

(GABPA monoclonal antibody, Western Blot analysis of GABPA expression in K-562.)

Western Blot (WB)

(Western Blot analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody. Lane 1: GABPA transfected lysate (51.3kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged GABPA is ~0.03ng/ml as a capture antibody.)

Related Product Information for anti-GABPA antibody

GA binding protein transcription factor, alpha subunit 60kD. It is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, it may play a role in the Down Syndrome phenotype.

Product Categories/Family for anti-GABPA antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 34192838
• NCBI GeneID: 2551
• Molecular Weight: 51,295 Da
• NCBI Official Full Name: Homo sapiens GA binding protein transcription factor, alpha subunit 60kDa, mRNA
• NCBI Official Synonym Full Names: GA binding protein transcription factor, alpha subunit 60kDa
• NCBI Official Symbol: GABPA
• NCBI Official Synonym Symbols: NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• NCBI Protein Information: GA-binding protein alpha chain; GA binding protein transcription factor alpha subunit 60kDa; GABP subunit alpha; human nuclear respiratory factor-2 subunit alpha; nuclear respiratory factor 2 alpha subunit; nuclear respiratory factor 2 subunit alpha; tran
• Protein Family: GA-binding protein

NCBI Description

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2010]