Goat DORFIN/RNF19 Polyclonal Antibody | anti-RNF19A antibody

Goat anti-DORFIN/RNF19 Antibody

Cat. Num. AAA421150

• Gene Names: RNF19A; RNF19
• Reactivity: Expected from sequence similarity: Human, Dog, Pig, Cow
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: DORFIN/RNF19; Polyclonal Antibody; Goat anti-DORFIN/RNF19 Antibody; RNF19A; DORFIN; dorfin; DKFZp566B1346; ring finger protein 19; p38 protein; double ring-finger protein; Dorfin; ring-IBR-ring domain containing protein Dorfin; RNF19; RING finger protein 19 isoform; protein p38 interacting with transcription factor Sp1; RNF19A antibody; DORFIN antibody; dorfin antibody; DKFZp566B1346 antibody; ring finger protein 19 antibody; p38 protein antibody; Dorfin antibody; ring-IBR-ring domain containing protein Dorfin antibody; RNF19 antibody; RING finger protein 19 isoform antibody; protein p38 interacting with transcription factor Sp1 antibody; ring finger protein 19A antibody; DORFIN / RNF19; anti-RNF19A antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QTMELKVAIQTEI
• Sequence Length: 838

• Applicable Applications for anti-RNF19A antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: No signal obtained yet but low background observed in Human Skeletal Muscle, Human Kidney, Human Liver, Human Heart and Human Brain lysates at up to 2ug/ml.
• Immunogen: Peptide with sequence C-QTMELKVAIQTEI, from the C Terminus of the protein sequence according to NP_056250.3; NP_904355.1.
• Epitope: C Terminus
• Note: Both variants (NP_056250.3; NP_904355.1) encode the same protein.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 35493782
• NCBI GeneID: 25897
• NCBI Accession #: NP_056250.3; NP_904355.1
• NCBI GenBank Nucleotide #: NM_015435.4
• Molecular Weight: 87,256 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF19A
• NCBI Official Synonym Full Names: ring finger protein 19A, RBR E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF19A
• NCBI Official Synonym Symbols: RNF19
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF19A
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF19A
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF19A
• UniProt Synonym Gene Names: RNF19; Dorfin
• UniProt Entry Name: RN19A_HUMAN

NCBI Description

This gene encodes a member of the ring between ring fingers (RBR) protein family, and the encoded protein contains two RING-finger motifs and an in between RING fingers motif. This protein is an E3 ubiquitin ligase that is localized to Lewy bodies, and ubiquitylates synphilin-1, which is an interacting protein of alpha synuclein in neurons. The encoded protein may be involved in amyotrophic lateral sclerosis and Parkinson's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

RNF19: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection. Belongs to the RBR family. RNF19 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Membrane protein, multi-pass; EC 6.3.2.-; EC 6.3.2.19; Transcription, coactivator/corepressor; Membrane protein, integral; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: centrosome; cytoplasm; cytosol; integral to membrane; ubiquitin ligase complex

Molecular Function: ligase activity; transcription factor binding; ubiquitin conjugating enzyme binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: microtubule cytoskeleton organization and biogenesis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process