Goat CSK Polyclonal Antibody | anti-CSK antibody

Goat anti-CSK Antibody

Cat. Num. AAA421145

• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CSK; Polyclonal Antibody; Goat anti-CSK Antibody; c-src tyrosine kinase; MGC117393; CSK antibody; c-src tyrosine kinase antibody; MGC117393 antibody; anti-CSK antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EQLEHIKTHELHL
• Sequence Length: 450

• Applicable Applications for anti-CSK antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Colon shows staining of a cell layer between the mucosal columns and the muscularis mucosae. Recommended concentration, 3-6ug/ml.; Western Blot: Approx 50kDa band observed in lysates of cell line Jurkat and in Mouse and Rat Spleen lysates (calculated MW of 50.7kDa according to NP_004374.1). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-EQLEHIKTHELHL, from the C Terminus of the protein sequence according to NP_004374.1; NP_001120662.1.
• Epitope: C Terminus
• Note: Reported variants represent identical protein: NP_004374.1; NP_001120662.1.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 4758078
• NCBI GeneID: 1445
• NCBI Accession #: NP_004374.1; NP_001120662.1
• NCBI GenBank Nucleotide #: NM_004383.2
• Molecular Weight: 50,704 Da
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: c-src tyrosine kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK
• UniProt Entry Name: CSK_HUMAN

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; intercellular junction; lipid raft; plasma membrane

Molecular Function: ATP binding; identical protein binding; kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein C-terminus binding; protein phosphatase binding; protein-tyrosine kinase activity; receptor binding

Biological Process: adaptive immune response; brain development; cell differentiation; cell migration; central nervous system development; epidermal growth factor receptor signaling pathway; innate immune response; negative regulation of bone resorption; negative regulation of cell proliferation; negative regulation of Golgi to plasma membrane protein transport; negative regulation of interleukin-6 production; negative regulation of kinase activity; negative regulation of phagocytosis; oligodendrocyte differentiation; positive regulation of MAP kinase activity; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway