Goat CENTB2/ACAP2 Polyclonal Antibody | anti-ACAP2 antibody

Goat anti-CENTB2/ACAP2 Antibody

Cat. Num. AAA421076

• Gene Names: ACAP2; CENTB2; CNT-B2
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CENTB2/ACAP2; Polyclonal Antibody; Goat anti-CENTB2/ACAP2 Antibody; ACAP2; CENTB2; centaurin; beta 2; CNT-B2; KIAA0041; centaurin beta2; Arf GAP with coiled coil; ANK repeat and PH domains 2; ArfGAP with coiled-coil; ankyrin repeat and PH domains 2; ACAP2 antibody; CENTB2 antibody; beta 2 antibody; CNT-B2 antibody; KIAA0041 antibody; centaurin beta2 antibody; ANK repeat and PH domains 2 antibody; ankyrin repeat and PH domains 2 antibody; CENTB2 / ACAP2; anti-ACAP2 antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EKLNRFQQDSQKF
• Sequence Length: 778

• Applicable Applications for anti-ACAP2 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10ug/ml.; Western Blot: Approx 90kDa band observed in Mouse and Rat Lung lysates and in lysates of cell lines MOLT4 and NIH3T3 (calculated MW of 88.1kDa according to Human NP_036419 and 87.2kDa according to Mouse NP_084414.1 and Rat NP_001029178.1). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-EKLNRFQQDSQKF, from the C Terminus of the protein sequence according to NP_036419.3.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0.3ug/ml) staining of Mouse (A) and Rat (B) Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0.3ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 187608516
• NCBI GeneID: 23527
• NCBI Accession #: NP_036419.3
• NCBI GenBank Nucleotide #: NM_012287.5
• Molecular Weight: 88,029 Da
• NCBI Official Full Name: arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
• NCBI Official Synonym Full Names: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
• NCBI Official Symbol: ACAP2
• NCBI Official Synonym Symbols: CENTB2; CNT-B2
• NCBI Protein Information: arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
• UniProt Protein Name: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
• Protein Family: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ACAP2
• UniProt Synonym Gene Names: CENTB2; KIAA0041; Cnt-b2
• UniProt Entry Name: ACAP2_HUMAN

Uniprot Description

CENTB2: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins. Regulates cytoskeletal and membrane trafficking events in the cell periphery. Binding of phosphoinositides to a pleckstrin homology domain may regulate subcellular localization and activity.

Protein type: GAPs, ARF; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: endosome membrane; membrane

Molecular Function: GTPase activator activity; metal ion binding; Rab GTPase binding

Biological Process: positive regulation of GTPase activity