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Western Blot (WB) ((1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat VTI1B Polyclonal Antibody | anti-VTI1B antibody

Goat anti-VTI1B Antibody

Gene Names
VTI1B; VTI1; VTI2; VTI1L; v-SNARE; vti1-rp1; VTI1-LIKE
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
VTI1B; Polyclonal Antibody; Goat anti-VTI1B Antibody; vesicle transport through interaction with t-SNAREs homolog 1B (yeast); HGNC: 17793; VTI1; VTI1L; VTI2; v-SNARE; vesicle transport through interaction with t-SNAREs 1B; vesicle-associated soluble NSF attachment protein receptor; VTI1B antibody; vesicle transport through interaction with t-SNAREs homolog 1B (yeast) antibody; HGNC: 17793 antibody; VTI1 antibody; VTI1L antibody; VTI2 antibody; v-SNARE antibody; vesicle transport through interaction with t-SNAREs 1B antibody; vesicle-associated soluble NSF attachment protein receptor antibody; anti-VTI1B antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RDFDEKQQEANET
Sequence Length
232
Applicable Applications for anti-VTI1B antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 26-28kDa band observed in Human Duodenum, Kidney and Testes lysates (calculated MW of 26.7kDa according to NP_006361.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-RDFDEKQQEANET, from the internal region of the protein sequence according to NP_006361.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,810 Da
NCBI Official Full Name
vesicle transport through interaction with t-SNAREs homolog 1B
NCBI Official Synonym Full Names
vesicle transport through interaction with t-SNAREs 1B
NCBI Official Symbol
VTI1B
NCBI Official Synonym Symbols
VTI1; VTI2; VTI1L; v-SNARE; vti1-rp1; VTI1-LIKE
NCBI Protein Information
vesicle transport through interaction with t-SNAREs homolog 1B
UniProt Protein Name
Vesicle transport through interaction with t-SNAREs homolog 1B
UniProt Gene Name
VTI1B
UniProt Synonym Gene Names
VTI1; VTI1L; VTI1L1; VTI2
UniProt Entry Name
VTI1B_HUMAN

Uniprot Description

VTI1B: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence. Belongs to the VTI1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: cell soma; cytoplasm; cytosol; endoplasmic reticulum membrane; Golgi apparatus; integral to membrane; intracellular membrane-bound organelle; late endosome membrane; lysosomal membrane; perinuclear region of cytoplasm; recycling endosome; SNARE complex; synaptic vesicle; vesicle

Molecular Function: chloride channel inhibitor activity; protein binding; SNAP receptor activity; SNARE binding

Biological Process: cell proliferation; ER to Golgi vesicle-mediated transport; Golgi to vacuole transport; intra-Golgi vesicle-mediated transport; protein targeting to vacuole; retrograde transport, endosome to Golgi; vesicle docking during exocytosis; vesicle fusion with Golgi apparatus; vesicle-mediated transport

Research Articles on VTI1B

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Product Notes

The VTI1B vti1b (Catalog #AAA420977) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-VTI1B Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's VTI1B can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 26-28kDa band observed in Human Duodenum, Kidney and Testes lysates (calculated MW of 26.7kDa according to NP_006361.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the VTI1B vti1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RDFDEKQQEA NET. It is sometimes possible for the material contained within the vial of "VTI1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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