Western Blot (WB)
((2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Sak/STK18/PLK4 Polyclonal Antibody | anti-PLK4 antibody
Goat anti-Sak/STK18/PLK4 Antibody
Western blot: Approx 110kDa band observed in Human Colon lysates (calculated MW of 109kDa according to NP_055079.3). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Western Blot (WB)
((2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunofluorescence (IF)
(MBS420966 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
(MBS420966 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
(MBS420966 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
(MBS420966 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Flow Cytometry (FC)
(MBS420966 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC)
(MBS420966 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Uniprot Description
PLK4: a kinase of the PLK family. Localizes to centrosomes and the cleavage furrow during cytokinesis. Interacts with the protein kinase Tec.
Protein type: EC 2.7.11.21; Protein kinase, Other; Nucleolus; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family
Chromosomal Location of Human Ortholog: 4q28
Cellular Component: centriole; centrosome; cleavage furrow; cytosol; nucleolus; XY body
Molecular Function: ATP binding; identical protein binding; protein binding; protein serine/threonine kinase activity
Biological Process: centriole replication; G2/M transition of mitotic cell cycle; positive regulation of centriole replication; protein amino acid phosphorylation
Disease: Microcephaly And Chorioretinopathy, Autosomal Recessive, 2
Research Articles on PLK4
Similar Products
Product Notes
The PLK4 plk4 (Catalog #AAA420966) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Sak/STK18/PLK4 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Sak/STK18/PLK4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 110kDa band observed in Human Colon lysates (calculated MW of 109kDa according to NP_055079.3). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the PLK4 plk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ATCIGEKIED FKVGN. It is sometimes possible for the material contained within the vial of "Sak/STK18/PLK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
