Goat EMI2 Polyclonal Antibody | anti-FBXO43 antibody

Goat anti-EMI2 Antibody

Cat. Num. AAA420772

• Gene Names: FBXO43; EMI2; ERP1; FBX43
• Reactivity: Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: EMI2; Polyclonal Antibody; Goat anti-EMI2 Antibody; FBXO43; F-box protein 43; Fbx43; early mitotic inhibitor 2; ERP1; FBXO43 antibody; EMI2 antibody; F-box protein 43 antibody; Fbx43 antibody; early mitotic inhibitor 2 antibody; ERP1 antibody; anti-FBXO43 antibody

• Host: Goat
• Reactivity: Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PGSAQSKRNLKRL
• Sequence Length: 708

• Applicable Applications for anti-FBXO43 antibody: Peptide ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: This product has been successfully used on Rat Pachytene Spermatocytes showing a 71kDa band (Perrard et al, PLoS One.2009 Oct 5;4(10): e7237. PMID: 19802389) and in Mouse Oocytes (Bernhardt et al, Biol Reprod.2012 Apr 19;86(4): 114. PMID: 22302686). Recommended concentration: 1-3ug/ml.; IF/FC: This product has been successfully used in Immunofluorescence and Flow Cytometry on Rat meiotic Testis cells: (Perrard et al, PLoS One.2009 Oct 5;4(10): e7237. PMID: 19802389). Recommended concentration: 5-10ug/ml.
• Immunogen: Peptide with sequence C-PGSAQSKRNLKRL, from the C Terminus of the protein sequence according to NP_001025031.2.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 117606351
• NCBI GeneID: 286151
• NCBI Accession #: NP_001025031.2
• NCBI GenBank Nucleotide #: NM_001029860.3
• Molecular Weight: 78,402 Da
• NCBI Official Full Name: F-box only protein 43
• NCBI Official Synonym Full Names: F-box protein 43
• NCBI Official Symbol: FBXO43
• NCBI Official Synonym Symbols: EMI2; ERP1; FBX43
• NCBI Protein Information: F-box only protein 43
• UniProt Protein Name: F-box only protein 43
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO43
• UniProt Synonym Gene Names: EMI2
• UniProt Entry Name: FBX43_HUMAN

NCBI Description

Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]

Uniprot Description

FBX43: Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (Probable). Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with CDC20.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: nucleus

Molecular Function: metal ion binding

Biological Process: meiotic cell cycle; negative regulation of meiosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein ubiquitination; regulation of mitosis