Western Blot (WB)
((0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat MURF2/TRIM55 Polyclonal Antibody | anti-TRIM55 antibody
Goat anti-MURF2/TRIM55 Antibody
Gene Names
TRIM55; RNF29; muRF2; MURF-2
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MURF2/TRIM55; Polyclonal Antibody; Goat anti-MURF2/TRIM55 Antibody; TRIM55; RNF29; MURF-2; ring finger protein 29; for muscle specific ring finger 2; tripartite motif-containing 55; muscle specific ring finger 2; TRIM55 antibody; RNF29 antibody; MURF-2 antibody; ring finger protein 29 antibody; for muscle specific ring finger 2 antibody; tripartite motif-containing 55 antibody; muscle specific ring finger 2 antibody; MURF2 / TRIM55; anti-TRIM55 antibody
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HIFSFSWLNSLNE
Sequence Length
548
Applicable Applications for anti-TRIM55 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 85kDa band observed in Human and Mouse Heart lysates (calculated MW of 60.5kDa according to NP_908973.1). The observed molecular weight corresponds to earlier findings in literature with the same antibody (Willis et al, Circ Res.2007 Mar 2;100(4): 456-9. PMID: 17272810). Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 85kDa band observed in Human and Mouse Heart lysates (calculated MW of 60.5kDa according to NP_908973.1). The observed molecular weight corresponds to earlier findings in literature with the same antibody (Willis et al, Circ Res.2007 Mar 2;100(4): 456-9. PMID: 17272810). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-HIFSFSWLNSLNE, from the C Terminus of the protein sequence according to NP_908973.1; NP_908974.1; NP_908975.1.
Epitope
C Terminus
Note
This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp. ).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,841 Da
NCBI Official Full Name
tripartite motif-containing protein 55 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 55
NCBI Official Symbol
TRIM55
NCBI Official Synonym Symbols
RNF29; muRF2; MURF-2
NCBI Protein Information
tripartite motif-containing protein 55
UniProt Protein Name
Tripartite motif-containing protein 55
Protein Family
UniProt Gene Name
TRIM55
UniProt Synonym Gene Names
MURF2; RNF29; MuRF-2; MuRF2
UniProt Entry Name
TRI55_HUMAN
NCBI Description
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein associates transiently with microtubules, myosin, and titin during muscle sarcomere assembly. It may act as a transient adaptor and plays a regulatory role in the assembly of sarcomeres. Four alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
TRIM55: May regulate gene expression and protein turnover in muscle cells. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 8q13.1
Cellular Component: cytoplasm; microtubule; nucleus
Molecular Function: identical protein binding; protein binding; signal transducer activity; zinc ion binding
Biological Process: signal transduction
Research Articles on TRIM55
Similar Products
Product Notes
The TRIM55 trim55 (Catalog #AAA420662) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MURF2/TRIM55 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's MURF2/TRIM55 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 85kDa band observed in Human and Mouse Heart lysates (calculated MW of 60.5kDa according to NP_908973.1). The observed molecular weight corresponds to earlier findings in literature with the same antibody (Willis et al, Circ Res.2007 Mar 2;100(4): 456-9. PMID: 17272810). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the TRIM55 trim55 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HIFSFSWLNS LNE. It is sometimes possible for the material contained within the vial of "MURF2/TRIM55, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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