Western Blot (WB)
((1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PTCH Polyclonal Antibody | anti-PTCH antibody
Goat anti-PTCH (Internal) Antibody
Western Blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 161kDa according to NP_000255.1). Recommended concentration: 1-3ug/ml.
Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C3H10T1/2 cells and mouse embryonic primary cells.
Western Blot (WB)
((1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq, Jul 2008]
Uniprot Description
PTCH1: a multi-pass membrane protein member of the ?patched? family that acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog protein?s signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Interacts with SNX17. Expressed in tumor cells but not in normal skin. In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH1 are the cause of basal cell nevus syndrome (BCNS), also known as Gorlin syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas, fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 defects is also the cause of holoprosencephaly, the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres.
Protein type: Membrane protein, integral; Tumor suppressor; Membrane protein, multi-pass; Cell cycle regulation
Chromosomal Location of Human Ortholog: 9q22.3
Cellular Component: caveola; Golgi apparatus; integral to membrane; intracellular membrane-bound organelle; midbody; perinuclear region of cytoplasm; plasma membrane; postsynaptic density
Molecular Function: cholesterol binding; cyclin binding; hedgehog receptor activity; heparin binding; patched binding; protein binding; protein complex binding; smoothened binding
Biological Process: brain development; cell fate determination; dorsal/ventral pattern formation; embryonic limb morphogenesis; embryonic organ development; epidermal cell fate specification; glucose homeostasis; heart morphogenesis; hindlimb morphogenesis; in utero embryonic development; keratinocyte proliferation; limb morphogenesis; negative regulation of cell division; negative regulation of epithelial cell proliferation; negative regulation of multicellular organism growth; negative regulation of osteoblast differentiation; negative regulation of smoothened signaling pathway; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; neural plate pattern formation; neural tube closure; neural tube patterning; organ morphogenesis; pharyngeal system development; positive regulation of epidermal cell differentiation; positive regulation of transcription, DNA-dependent; protein processing; regulation of mitotic cell cycle; regulation of protein localization; regulation of smoothened signaling pathway; response to chlorate; response to drug; response to estradiol stimulus; response to mechanical stimulus; response to retinoic acid; smoothened signaling pathway; spinal cord motor neuron differentiation; ureteric bud branching
Disease: Basal Cell Carcinoma, Susceptibility To, 1; Basal Cell Nevus Syndrome; Holoprosencephaly 7
Research Articles on PTCH
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Product Notes
The PTCH ptch1 (Catalog #AAA420238) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PTCH (Internal) Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PTCH can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 161kDa according to NP_000255.1). Recommended concentration: 1-3ug/ml. Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C3H10T1/2 cells and mouse embryonic primary cells. Researchers should empirically determine the suitability of the PTCH ptch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HPESRHHPPS NPRQQ. It is sometimes possible for the material contained within the vial of "PTCH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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