Goat Atoh7 Polyclonal Antibody | anti-Atoh7 antibody
Goat anti-Atoh7 (mouse) Antibody
Gene Names
Atoh7; Math5; bHLHa13
Reactivity
Expected from sequence similarity: Mouse, Rat
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Atoh7; Polyclonal Antibody; Goat anti-Atoh7 (mouse) Antibody; atonal homolog 7 (Drosophila); Math; atonal homolog 7; Atoh7 antibody; atonal homolog 7 (Drosophila) antibody; Math antibody; atonal homolog 7 antibody; Atoh7 (mouse); anti-Atoh7 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CEQRGRDHPYLP
Sequence Length
149
Applicable Applications for anti-Atoh7 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave bands at approx 50kDa and 38kDa in Mouse Eye and Rat Optical Nerve lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 16.9kDa according to NP_058560.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Western Blot: Preliminary experiments gave bands at approx 50kDa and 38kDa in Mouse Eye and Rat Optical Nerve lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 16.9kDa according to NP_058560.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunogen
Peptide with sequence CEQRGRDHPYLP, from the internal region of the protein sequence according to NP_058560.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,569 Da
NCBI Official Full Name
protein atonal homolog 7
NCBI Official Synonym Full Names
atonal bHLH transcription factor 7
NCBI Official Symbol
Atoh7
NCBI Official Synonym Symbols
Math5; bHLHa13
NCBI Protein Information
protein atonal homolog 7
UniProt Protein Name
Protein atonal homolog 7
Protein Family
UniProt Gene Name
Atoh7
UniProt Synonym Gene Names
Ath5; mATH5
UniProt Entry Name
ATOH7_MOUSE
Uniprot Description
ATOH7: Transcription factor involved in the differentiation of retinal ganglion cells. Defects in ATOH7 are the cause of retinal non-attachment congenital non-syndromic (RNANC). A condition characterized by separation of the inner layers of the retina (neural retina) from the pigment epithelium. Clinical findings include lack of perception of light, massive retrolental mass, shallow anterior chamber, and nystagmus in otherwise normal individuals. RNANC is caused by a 6.5 kb deletion that spans a remote cis regulatory element 20 kb upstream from ATOH7.
Cellular Component: nucleus
Molecular Function: DNA binding; protein dimerization activity
Biological Process: cell differentiation; circadian rhythm; entrainment of circadian clock; multicellular organismal development; nervous system development; optic nerve development; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on Atoh7
1. Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate in ATOH7 deficient mice
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Product Notes
The Atoh7 atoh7 (Catalog #AAA420210) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Atoh7 (mouse) Antibody reacts with Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Atoh7 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave bands at approx 50kDa and 38kDa in Mouse Eye and Rat Optical Nerve lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 16.9kDa according to NP_058560.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Researchers should empirically determine the suitability of the Atoh7 atoh7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CEQRGRDHPY LP. It is sometimes possible for the material contained within the vial of "Atoh7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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