Western Blot (WB)
((0.3ug/ml) staining of human thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat GRAP Polyclonal Antibody | anti-GRAP antibody
Goat anti-GRAP Antibody
Western Blot: Approx 26kDa band observed in human thymus lysates (calculated MW of 25.3kDa according to NP_006604.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of human thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of human thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway. [provided by RefSeq, Jul 2012]
Uniprot Description
GRAP: Couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway. Belongs to the GRB2/sem-5/DRK family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 17p11.2
Cellular Component: cytoplasm; cytosol
Molecular Function: protein binding; SH3/SH2 adaptor activity
Biological Process: cell-cell signaling; positive regulation of signal transduction; Ras protein signal transduction
Research Articles on GRAP
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Product Notes
The GRAP grap (Catalog #AAA420198) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GRAP Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's GRAP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 26kDa band observed in human thymus lysates (calculated MW of 25.3kDa according to NP_006604.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the GRAP grap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GFFPRSYVQP VHL. It is sometimes possible for the material contained within the vial of "GRAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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