Goat HIP-55/SH3P7 Polyclonal Antibody | anti-DBNL antibody

Goat anti-HIP-55/SH3P7 Antibody

Cat. Num. AAA420195

• Gene Names: DBNL; ABP1; HIP55; SH3P7; HIP-55
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: HIP-55/SH3P7; Polyclonal Antibody; Goat anti-HIP-55/SH3P7 Antibody; DBNL; HIP-55; SH3P7; src homology 3 domain-containing protein HIP-55; HIP-55 protein; drebrin F; drebrin-like; ABP1; DBNL antibody; HIP-55 antibody; SH3P7 antibody; src homology 3 domain-containing protein HIP-55 antibody; HIP-55 protein antibody; drebrin F antibody; drebrin-like antibody; ABP1 antibody; HIP-55 / SH3P7; anti-DBNL antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: AANLSRNGPALQE
• Sequence Length: 431

• Applicable Applications for anti-DBNL antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Approx 55kDa band observed in Jurkat and 3T3 lysates (calculated MW of 49. 0kDa according to Human NP_001116428.1 and 48.7kDa according to Mouse NP_001139780.1). Recommended for use at 1-3ug/ml.
• Immunogen: Peptide with sequence AANLSRNGPALQE-C, from the N Terminus of the protein sequence according to NP_054782.2; NP_001014436.1; NP_001116428.1.
• Epitope: N Terminus
• Note: This antibody is expected to recognise all reported isoforms (NP_054782.2; NP_001014436.1; NP_001116428.1)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(Staining (1ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB)

((1ug/ml) staining of 3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 21361670
• NCBI GeneID: 28988
• NCBI Accession #: NP_054782.2; NP_001014436.1; NP_001116428.1
• NCBI GenBank Nucleotide #: NM_014063.6
• Molecular Weight: 43,041 Da
• NCBI Official Full Name: drebrin-like protein isoform a
• NCBI Official Synonym Full Names: drebrin like
• NCBI Official Symbol: DBNL
• NCBI Official Synonym Symbols: ABP1; HIP55; SH3P7; HIP-55
• NCBI Protein Information: drebrin-like protein
• UniProt Protein Name: Drebrin-like protein
• Protein Family: Drebrin-like protein
• UniProt Gene Name: DBNL
• UniProt Synonym Gene Names: CMAP; SH3P7; HIP-55
• UniProt Entry Name: DBNL_HUMAN

Uniprot Description

DBNL: an SH3-containing adaptor protein. Interacts with hematopoietic progenitor kinase 1a (HIPK1). It is recruited to glycolipid-enriched microdomains following T cell receptor (TCR) stimulation. Interacts with and is phosphorylated by ZAP-70 after TCR signaling. May regulate JNK activation following TCR signaling, presumably via its interaction with HIPKIt is a member of the drebrin family of proteins that are developmentally regulated in the brain.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: cell cortex; cell-cell adherens junction; cytoplasm; cytosol; lamellipodium; podosome; ruffle

Molecular Function: actin binding; actin filament binding; enzyme activator activity; protein binding

Biological Process: activation of JNK activity; neurite morphogenesis; Rac protein signal transduction; synaptogenesis