Western Blot (WB)
(Staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat PI31/PSMF1 Polyclonal Antibody | anti-PSMF1 antibody
Goat anti-PI31/PSMF1 (Isoform 1) Antibody
Western Blot: Approx 35kDa band observed in Human Kidney lysates (calculated MW of 29.8kDa according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approx.35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
(Staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing PSMF1 (RC218938) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing PSMF1 (RC218938) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PSMF1: Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28. Belongs to the proteasome inhibitor PI31 family.
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: cytosol; endoplasmic reticulum; membrane; nucleoplasm; proteasome core complex
Molecular Function: endopeptidase inhibitor activity; protein binding
Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway
Research Articles on PSMF1
Similar Products
Product Notes
The PSMF1 psmf1 (Catalog #AAA420105) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PI31/PSMF1 (Isoform 1) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PI31/PSMF1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 35kDa band observed in Human Kidney lysates (calculated MW of 29.8kDa according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approx.35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the PSMF1 psmf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DHLPPPGYDD MYL. It is sometimes possible for the material contained within the vial of "PI31/PSMF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
