Rabbit PSMF1 Polyclonal Antibody | anti-PSMF1 antibody

PSMF1 (Proteasome Inhibitor PI31 Subunit, PI31) (Biotin)

Cat. Num. AAA6433791

• Gene Names: PSMF1; PI31
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: PSMF1; Polyclonal Antibody; PSMF1 (Proteasome Inhibitor PI31 Subunit; PI31) (Biotin); anti-PSMF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PSMF1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PSMF1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PSMF1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PSMF1 antibody.)

Product Categories/Family for anti-PSMF1 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 145611426
• NCBI GeneID: 9491
• NCBI Accession #: NP_006805.2
• NCBI GenBank Nucleotide #: NM_006814.3
• UniProt Accession #: Q92530; Q9H4I1; A0AVQ9; D3DVW3
• Molecular Weight: 29,817 Da
• NCBI Official Full Name: proteasome inhibitor PI31 subunit
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
• NCBI Official Symbol: PSMF1
• NCBI Official Synonym Symbols: PI31
• NCBI Protein Information: proteasome inhibitor PI31 subunit; hPI31; proteasome inhibitor hP131 subunit
• UniProt Protein Name: Proteasome inhibitor PI31 subunit
• Protein Family: Proteasome inhibitor
• UniProt Gene Name: PSMF1
• UniProt Synonym Gene Names: hPI31
• UniProt Entry Name: PSMF1_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMF1: Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28. Belongs to the proteasome inhibitor PI31 family.

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: nucleoplasm; proteasome core complex; membrane; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: protein binding; endopeptidase inhibitor activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle