Goat PTPRM Polyclonal Antibody | anti-PTPRM antibody
Goat anti-PTPRM Antibody
Western Blot: Preliminary experiments gave bands at approx 110kDa and 16kDa in Rat Retina lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 165kDa according to Human NP_001098714.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP has been shown to mediate cell-cell aggregation through the interaction with another molecule of this PTP on an adjacent cell. This PTP can interact with scaffolding protein RACK1/GNB2L1, which may be necessary for the downstream signaling in response to cell-cell adhesion. Alternative splicing results in multiple transcripts encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
PTPRM: a single-pass type I membrane protein involved in cell-cell adhesion through homophilic interactions. A protein-tyrosine phosphatase receptor of the class 2B subfamily. A negative regulator of proliferation and invasion of breast cancer cells. The PTPRM gene is frequently hypermethylated in sporadic cancers. Modulates p120 catenin phosphorylation, promoting adipogenic differentiation. Two isoforms of the human protein have been reported.
Protein type: Receptor protein phosphatase, tyrosine; Membrane protein, integral; EC 3.1.3.48; Motility/polarity/chemotaxis; Phosphatase
Chromosomal Location of Human Ortholog: 18p11.2
Cellular Component: cell-cell adherens junction; cytoplasm; integral to plasma membrane; intercellular junction; lamellipodium; perinuclear region of cytoplasm
Molecular Function: cadherin binding; identical protein binding; protein binding; protein tyrosine phosphatase activity; transmembrane receptor protein tyrosine phosphatase activity
Biological Process: homophilic cell adhesion; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; neurite development; positive regulation of vasodilation; protein amino acid dephosphorylation; response to drug; retinal ganglion cell axon guidance; signal transduction
Research Articles on PTPRM
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Product Notes
The PTPRM ptprm (Catalog #AAA420071) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PTPRM Antibody reacts with Expected from sequence similarity: Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's PTPRM can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave bands at approx 110kDa and 16kDa in Rat Retina lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 165kDa according to Human NP_001098714.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Researchers should empirically determine the suitability of the PTPRM ptprm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KLIRQVDKWQ EEYN. It is sometimes possible for the material contained within the vial of "PTPRM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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