Western Blot (WB)
((0. 03ug/ml) staining of human heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat STAP2/BKS Polyclonal Antibody | anti-STAP2 antibody
Goat anti-STAP2/BKS Antibody
Immunohistochemistry: In paraffin embedded Human Brain (Cortex) shows textured cytoplasm staining in neuronal bodies and processes. Recommended concentration, 1-2ug/ml.
Western Blot: Approx 48kDa band observed in human heart lysates (calculated MW of 49.6kDa according to NP_060190.2). Recommended concentration: 0. 05-0.3ug/ml.
Western Blot (WB)
((0. 03ug/ml) staining of human heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of human heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
STAP2: a widely expressed proimflammatory adaptor protein that regulates immune and inflammatory responses. Binds and modulates a variety of molecules including Brk, CSFR, FCER1, and BCR-ABL. The interaction between STAP-2 and BCR-ABL appears to provide a growth advantage to BCR-ABL+ chronic myeloid leukemia cells. Suppression of STAP-2 expression in K562 CML cells suppressed tumor formation in mice. Enables intestinal proinflammatory responses in vivo: promotes macrophage recruitment in dextran sodium sulfate induced colitis, and promotes the ability of CXCL12-induced migration in macrophages. STAP-2 knockdown in T47D breast cancer cells induced a significant decrease of proliferation. Contains a pleckstrin homology, Src homology 2-like, and as well as a STAT3-binding domains. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cytosol; plasma membrane
Molecular Function: protein binding
Biological Process: positive regulation of tyrosine phosphorylation of Stat3 protein
Research Articles on STAP2
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Product Notes
The STAP2 stap2 (Catalog #AAA420067) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-STAP2/BKS Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's STAP2/BKS can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Brain (Cortex) shows textured cytoplasm staining in neuronal bodies and processes. Recommended concentration, 1-2ug/ml. Western Blot: Approx 48kDa band observed in human heart lysates (calculated MW of 49.6kDa according to NP_060190.2). Recommended concentration: 0. 05-0.3ug/ml. Researchers should empirically determine the suitability of the STAP2 stap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ELQKKLEKRR ALEH. It is sometimes possible for the material contained within the vial of "STAP2/BKS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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