Immunohistochemistry (IHC)
(MBS420040 (3ug/ml) staining of paraffin embedded human pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
Goat Pancreatic Polypeptide/PPY Polyclonal Antibody | anti-PPY antibody
Goat anti-Pancreatic Polypeptide/PPY Antibody
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
WB: Preliminary experiments gave an approx 35-38kDa band in Human Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5kDa according to NP_002713.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide. This antibody has been successfully used in WB on Human.
IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3µg/ml. Paraffin embedded Human Pancreas and Intestine. Recommended concentration: 5ug/ml. This antibody has been successfully used in IHC on Human.
IF: This antibody has been successfully used in IF on Human and on Rat.
Immunohistochemistry (IHC)
(MBS420040 (3ug/ml) staining of paraffin embedded human pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
Immunohistochemistry (IHC)
(MBS420040 (3ug/ml) staining of paraffin embedded human pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
Immunohistochemistry (IHC)
(MBS420040 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS420040 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS420040 (5ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS420040 (5ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
Uniprot Description
PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: cytoplasm; extracellular region; extracellular space
Molecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; receptor binding
Biological Process: cell-cell signaling; digestion; feeding behavior; neuropeptide signaling pathway; positive regulation of defense response to virus by host; protein secretion; regulation of appetite
Research Articles on PPY
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Product Notes
The PPY ppy (Catalog #AAA420040) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Pancreatic Polypeptide/PPY Antibody reacts with Tested: Human, Rat Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Pancreatic Polypeptide/PPY can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA, Western Blot (WB), Immunohistochemistry (IHC). ELISA: Antibody detection limit dilution 1:32000. WB: Preliminary experiments gave an approx 35-38kDa band in Human Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5kDa according to NP_002713.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide. This antibody has been successfully used in WB on Human. IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3µg/ml. Paraffin embedded Human Pancreas and Intestine. Recommended concentration: 5ug/ml. This antibody has been successfully used in IHC on Human. IF: This antibody has been successfully used in IF on Human and on Rat. Researchers should empirically determine the suitability of the PPY ppy for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pancreatic Polypeptide/PPY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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