Rabbit IL1RN Polyclonal Antibody | anti-IL1RN antibody

Anti-IL1RN Antibody

Cat. Num. AAA419977

• Gene Names: IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: IL1RN; Polyclonal Antibody; Anti-IL1RN Antibody; anti-IL1RN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using IL1RN antibody. Immunohistochemistry of paraffin-embedded human epididymis using IL1RN antibody at dilution of 1:200 (200x lens). Immunohistochemistry of paraffin-embedded mouse lung using IL1RN antibody at dilution of 1:200 (200x lens). Immunohistochemistry of paraffin-embedded rat pancreas using IL1RN antibody at dilution of 1:200 (400x lens). Immunofluorescence analysis of MCF7 cell using IL1RN antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 143

• Applicable Applications for anti-IL1RN antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:50- 1:200
• Immunogen: Recombinant protein of human IL1RN
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Related Product Information for anti-IL1RN antibody

The founding members of the interleukin-1 (IL-1) superfamily include pro-inflammatory cytokines IL-1+/- and IL-12, and a third protein that acts as an IL-1 receptor antagonist (IL-1RA). At least six similar proteins have been recently identified, including a homolog of IL-1RA (IL1F5). The three better-characterized proteins (IL-1a, IL-1b and IL-1RA) are mainly expressed in macrophages, monocytes, and dendritic cells. IL-1a and IL-1b act as potent inflammatory cytokines that help regulate host defense and immune responses (1). Binding of these pro-inflammatory cytokines to an IL-1 receptor recruits adapter proteins (such as IRAK) to the receptor. Phosphorylation of these adaptor proteins promotes downstream signaling cascades associated with the immune response (2). Altered expression of both IL-1a and IL-1b is associated with an extensive list of human disorders, including Alzheimer's disease, rheumatoid arthritis, psoriasis and various forms of cancer (3, 4). IL-1RA acts as an anti-inflammatory cytokine, binding the IL-1 receptor to limit the response to inflammation (5). Because it plays a key role in regulating the inflammatory response, recombinant IL-1RA is a therapeutic agent used in the treatment of diseases such as rheumatoid arthritis. Alternatively, mutation of the corresponding IL-1RA gene may be associated with susceptibility to the development of specific cancers (6).

NCBI and Uniprot Product Information

• NCBI GI #: 974576772
• NCBI GeneID: 3557
• NCBI Accession #: NP_001305843.1
• NCBI GenBank Nucleotide #: NM_001318914.1
• UniProt Accession #: P18510; Q14628; Q53SC2; Q7RTZ4; Q96GD6; Q9UPC0; A8K4G1
• Molecular Weight: 16,142 Da
• NCBI Official Full Name: interleukin-1 receptor antagonist protein isoform 4
• NCBI Official Synonym Full Names: interleukin 1 receptor antagonist
• NCBI Official Symbol: IL1RN
• NCBI Official Synonym Symbols: DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
• NCBI Protein Information: interleukin-1 receptor antagonist protein
• UniProt Protein Name: Interleukin-1 receptor antagonist protein
• Protein Family: Interleukin-1 receptor antagonist protein
• UniProt Gene Name: IL1RN
• UniProt Synonym Gene Names: IL1F3; IL1RA; IL-1RN; IL-1ra; IRAP

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jan 2016]

Uniprot Description

IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 2q14.1

Cellular Component: extracellular space; plasma membrane

Molecular Function: cytokine activity; interleukin-1 receptor antagonist activity; interleukin-1 Type I receptor antagonist activity; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type I receptor binding; interleukin-1, Type II receptor binding; protein binding

Biological Process: negative regulation of heterotypic cell-cell adhesion; response to glucocorticoid stimulus

Disease: Gastric Cancer, Hereditary Diffuse; Microvascular Complications Of Diabetes, Susceptibility To, 4; Osteomyelitis, Sterile Multifocal, With Periostitis And Pustulosis