Rabbit ANGPT2 Polyclonal Antibody | anti-ANGPT2 antibody

Anti-ANGPT2 Antibody

Cat. Num. AAA419464

• Gene Names: ANGPT2; ANG2; AGPT2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: ANGPT2; Polyclonal Antibody; Anti-ANGPT2 Antibody; anti-ANGPT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of HepG2 cell lysate using ANGPT2 antibody.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 444

• Applicable Applications for anti-ANGPT2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200
• Immunogen: Recombinant protein of human ANGPT2
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Related Product Information for anti-ANGPT2 antibody

Angiopoietins are a family of Tie receptor ligands. There are four angiopoietins discovered so far: angiopoietins 1, 2, 3 and 4 (Ang1, 2, 3, and 4) (1-3). Ang1 binds to the Tie-2 receptor and leads to its autophosphorylation and subsequent activation of downstream signaling pathways. It plays an important role in blood vessel formation, maturation and subsequent stabilization (1, 4,5). Ang2 is an endothelium-specific growth factor that functions as an antagonist to Ang1, promotes vascular associated proinflammatory function, destabilizes quiescent endothelium, leads to vascular leakage and vascular destablization and remodeling (2, 6, 7). Ang2 is selectively expressed in many tumor tissues where, combined with other growth factors such as VEGF, it can promote vascular remodeling, angiogenesis and inflammation (7-9).

NCBI and Uniprot Product Information

• NCBI GI #: 169646754
• NCBI GeneID: 285
• NCBI Accession #: NP_001112360.1
• NCBI GenBank Nucleotide #: NM_001118888.1
• UniProt Accession #: O15123; Q9NRR7; Q9P2Y7; A0AV38; A8K205; B7ZLM7
• Molecular Weight: 56,848 Da
• NCBI Official Full Name: angiopoietin-2 isoform c
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: ANGPT2
• NCBI Official Synonym Symbols: ANG2; AGPT2
• NCBI Protein Information: angiopoietin-2
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT2
• UniProt Synonym Gene Names: ANG-2

NCBI Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular region; extracellular space

Molecular Function: protein binding; receptor binding; receptor tyrosine kinase binding

Biological Process: leukocyte migration; negative regulation of blood vessel endothelial cell migration; negative regulation of positive chemotaxis; signal transduction; Tie receptor signaling pathway