Rabbit anti-Human FBXW8 Polyclonal Antibody | anti-FBXW8 antibody

Anti-FBXW8 Antibody

Cat. Num. AAA416697

• Gene Names: FBXW8; FBW6; FBW8; FBX29; FBXW6; FBXO29
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: FBXW8; Polyclonal Antibody; Anti-FBXW8 Antibody; anti-FBXW8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 4673

• Applicable Applications for anti-FBXW8 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA titer: 1:160,000; Western blot analysis: 1:200-1000; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-FBXW8 antibody

This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

References

Cenciarelli,C.,Chiaur,D.S.,Guardavaccaro,D et,al Identification of a family of human F-box proteins. Curr. Biol. 1999;9 (20),:1177-1179

NCBI and Uniprot Product Information

• NCBI GI #: 30795120
• NCBI GeneID: 26259
• NCBI Accession #: NP_036306.1
• NCBI GenBank Nucleotide #: NM_012174
• Molecular Weight: 60,705 Da
• NCBI Official Full Name: Homo sapiens F-box and WD repeat domain containing 8 (FBXW8), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: F-box and WD repeat domain containing 8
• NCBI Official Symbol: FBXW8
• NCBI Official Synonym Symbols: FBW6; FBW8; FBX29; FBXW6; FBXO29
• NCBI Protein Information: F-box/WD repeat-containing protein 8; F-box and WD-40 domain protein 8; F-box and WD-40 domain-containing protein 8; F-box only protein 29
• UniProt Protein Name: F-box/WD repeat-containing protein 8
• Protein Family: F-box/WD repeat-containing protein
• UniProt Gene Name: FBXW8
• UniProt Entry Name: FBXW8_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXW8: Substrate-recognition component of a SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Cell development/differentiation

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; SCF ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: cell proliferation; protein ubiquitination; positive regulation of dendrite morphogenesis; Golgi organization and biogenesis