CAMKK1 active protein

Recombinant Human CAMKK1 (N-GST tag)

Cat. Num. AAA4160057

• Gene Names: CAMKK1; CAMKKA
• Purity: 70%-90%
• Synonyms: CAMKK1; Recombinant Human CAMKK1 (N-GST tag); CAMKKA; MGC34095; DKFZp761M0423; CAMKK1 active protein

• Host: Insect Cells
• Purity/Purification: 70%-90%
• Form/Format: Liquid; 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 505

• Bioactivity: The specific activity of CAMKK1 was determined to be 10 nmol/min/mg as per activity assay protocol.
• Preparation and Storage: Store product at-70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Related Product Information for CAMKK1 active protein

CAMKK1 or CAMKKa is a Ca(2+)/calmodulin-dependent protein kinase that activates CaM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively. Recent studies have shown that the activity of CAMKK1 is decreased upon phosphorylation by cAMP-dependent protein kinase (PKA) The CAMKKalpha has been identified in intact cells as AMPKKs, predicting a significant role for this kinase in regulating AMPK activity in vivo. It has been shown that 2-deoxyglucose-and ionomycin-stimulated AMPK activity is substantially reduced in HeLa cells transfected with small interfering RNAs specific for CAMKKa.

NCBI and Uniprot Product Information

• NCBI GI #: 14150045
• NCBI GeneID: 84254
• NCBI GenBank Nucleotide #: NM_032294
• UniProt Accession #: Q8N5S9
• Molecular Weight: 94.0 kDa
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase kinase 1 isoform a
• NCBI Official Synonym Full Names: calcium/calmodulin dependent protein kinase kinase 1
• NCBI Official Symbol: CAMKK1
• NCBI Official Synonym Symbols: CAMKKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase kinase 1
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase kinase 1
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMKK1
• UniProt Synonym Gene Names: CAMKKA; CaM-KK 1; CaM-kinase kinase 1; CaMKK 1; CaM-KK alpha; CaM-kinase kinase alpha; CaMKK alpha
• UniProt Entry Name: KKCC1_HUMAN

NCBI Description

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMKK1: a calcium/calmodulin-dependent S/T protein kinase of the CAMKK family. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death. 2 isoforms of the human protein are produced by alternative splicing. The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition. Binding of calmodulin may releave intrasteric autoinhibition. May be regulated through phosphorylation by CAMK1 and CAMK4. Appears to be autophosphorylated in a Ca2+/calmodulin-dependent manner. Phosphorylation of Ser-458 is blocked upon binding to Ca2+/calmodulin.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; EC 2.7.11.17; Other group; CAMKK family; Meta subfamily

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: nucleus; cytosol

Molecular Function: calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: synaptic transmission; protein amino acid phosphorylation