Testing Data
Rabbit anti-Human Aurora Kinase B Polyclonal Antibody | anti-AURKB antibody
Anti-Human Aurora Kinase B Antibody
Gene Names
AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Aurora Kinase B; Polyclonal Antibody; Anti-Human Aurora Kinase B Antibody; Human Aurora B; anti-AURKB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Aurora Kinase B protein. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.
Purity/Purification
Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
344
Applicable Applications for anti-AURKB antibody
Western Blot (WB), ELISA (EIA)
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. Human brain tissue lysate or HeLa cell lysate can be used as a positive control.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 227-238 of Human Aurora Kinase B protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-AURKB antibody
Aurora Kinase B (also known as Aurora-and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Aurora kinase B
NCBI Official Synonym Full Names
aurora kinase B
NCBI Official Symbol
AURKB
NCBI Official Synonym Symbols
AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
NCBI Protein Information
aurora kinase B
Protein Family
NCBI Description
This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
Research Articles on AURKB
Similar Products
Product Notes
The AURKB (Catalog #AAA4158630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human Aurora Kinase B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aurora Kinase B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. Human brain tissue lysate or HeLa cell lysate can be used as a positive control. Researchers should empirically determine the suitability of the AURKB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aurora Kinase B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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