Testing Data
Rabbit anti-Human IL-6 Polyclonal Antibody | anti-IL-6 antibody
Anti-Human IL-6 Antibody
Gene Names
Il6; ILg6; Ifnb2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified
Synonyms
IL-6; Polyclonal Antibody; Anti-Human IL-6 Antibody; Human IL-6; anti-IL-6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (i.e. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antibody is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antibody for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.
Purity/Purification
Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
211
Applicable Applications for anti-IL-6 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
This antibody has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. It recognizes IL-6. Reactivity in other immunoassays is unknown. For immunoblotting, use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human plus 10 ng/mL E.coli LPS. For immunohistochemistry, either paraffin fix or cryofix tissue. For immunoprecipitation, preclearing with a non-specific rabbit IgG is helpful to reduce background. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as the capture antibody.
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6
UniProt Protein Name
Interleukin-6
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_RAT
NCBI Description
a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
Uniprot Description
Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance
By similarity.
Subcellular location: Secreted.
Sequence similarities: Belongs to the IL-6 superfamily.
Research Articles on IL-6
Similar Products
Product Notes
The IL-6 il6 (Catalog #AAA4156810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human IL-6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). This antibody has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. It recognizes IL-6. Reactivity in other immunoassays is unknown. For immunoblotting, use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human plus 10 ng/mL E.coli LPS. For immunohistochemistry, either paraffin fix or cryofix tissue. For immunoprecipitation, preclearing with a non-specific rabbit IgG is helpful to reduce background. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as the capture antibody. Researchers should empirically determine the suitability of the IL-6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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