Testing Data
Rabbit anti-Mouse, Rat Hif3a Polyclonal Antibody | anti-Hif3a antibody
Anti-Mouse Hif3a Antibody
Reactivity
Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Hif3a; Polyclonal Antibody; Anti-Mouse Hif3a Antibody; Mouse Hif3a; anti-Hif3a antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This affinity-purified antibody is directed against mouse Hif3a protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Hif3 a protein from mouse (100% homology) and rat (91% homology). Reactivity against human Hif3 a is not expected as only 75% homology for the immunizing sequence is noted. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known.
Purity/Purification
Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Hif3a antibody
Western Blot (WB), ELISA (EIA)
Application Notes
This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 a protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 a due to sequence homology.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 581-592 of mouse Hif3 a (Hypoxia Inducible Factor).
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-Hif3a antibody
One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits (HIF1-a, HIF2-a, or HIF3-a) bound to the aryl hydrocarbon receptor nuclear translocator (ARNT) that is also known as HIF1-b. HIF-a is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and angiogenesis. Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxia-inducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as CREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.
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Product Notes
The Hif3a (Catalog #AAA4156762) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse Hif3a Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hif3a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 a protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 a due to sequence homology. Researchers should empirically determine the suitability of the Hif3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hif3a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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