Testing Data
Mouse anti-Human Keratin Monoclonal Antibody | anti-KRT16 antibody
Anti-Keratin (pan) Antibody
Gene Names
KRT16; K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity Purified
Synonyms
Keratin; Monoclonal Antibody; Anti-Keratin (pan) Antibody; Keratin (pan); anti-KRT16 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Specificity
This protein A purified mouse monoclonal antibody reacts specifically with keratins from human tissues and derived cell lines. This antibody reacts with keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa) and keratin 19 (40 kDa) derived from humans. Cross reactivity with keratins from other sources has not been determined. No reaction is expected against other filament proteins including vimentin, desmin and neurofilament protein.
Purity/Purification
Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
244
Applicable Applications for anti-KRT16 antibody
Immunohistochemistry (IHC)
Application Notes
This antibody is suitable for ELISA, immunohistochemistry, immunoblotting and immunoprecipitation. For a positive control use skin, colon carcinoma and squamous granulocyte carcinoma cells.
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for anti-KRT16 antibody
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) or type II (basic polypeptides). At least one member of both the acidic and basic families are expressed in epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Anti-Keratin (pan) reacts with a variety of keratins (4, 5, 6, 8, 10, 13 and 18) in a variety of normal and neoplastic epithelial. This antibody is also useful to stain cultured epithelial cells. This antibody reacts with simple epithelium and both basal and superbasal layers of squamous epithelium. Anti-keratin (pan) is useful to differentiate epithelial tumors from non-epithelial tumors. Keratins have a cytoplasmic localization.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
keratin, partial
NCBI Official Synonym Full Names
keratin 16
NCBI Official Symbol
KRT16
NCBI Official Synonym Symbols
K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
NCBI Protein Information
keratin, type I cytoskeletal 16
Protein Family
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]
Research Articles on KRT16
Similar Products
Product Notes
The KRT16 (Catalog #AAA4156679) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Keratin (pan) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). This antibody is suitable for ELISA, immunohistochemistry, immunoblotting and immunoprecipitation. For a positive control use skin, colon carcinoma and squamous granulocyte carcinoma cells. Researchers should empirically determine the suitability of the KRT16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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