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Western Blot (WB)

Rabbit MPG Polyclonal Antibody | anti-MPG antibody

Anti-MPG Antibody

Gene Names
MPG; AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
MPG; Polyclonal Antibody; Anti-MPG Antibody; anti-MPG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Western blot analysis of extracts of various cell lines, using MPG antibody. Immunofluorescence analysis of U20S cell using MPG antibody. Blue: DAPI for nuclear staining.
Purity/Purification
Affinity Purification
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence Length
293
Applicable Applications for anti-MPG antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50- 1:200
IF: 1:50- 1:100
IP: 1:50- 1:100
Immunogen
Recombinant protein of human MPG
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Western Blot (WB)

Immunofluorescence (IF)

Immunofluorescence (IF)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
30,824 Da
NCBI Official Full Name
MPG, partial
NCBI Official Synonym Full Names
N-methylpurine DNA glycosylase
NCBI Official Symbol
MPG
NCBI Official Synonym Symbols
AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1
NCBI Protein Information
DNA-3-methyladenine glycosylase
UniProt Protein Name
DNA-3-methyladenine glycosylase
UniProt Gene Name
MPG
UniProt Synonym Gene Names
AAG; ANPG; MID1

Uniprot Description

MPG: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Belongs to the DNA glycosylase MPG family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.2.21; Hydrolase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm

Molecular Function: alkylbase DNA N-glycosylase activity; damaged DNA binding; DNA N-glycosylase activity; protein binding

Biological Process: base-excision repair; depurination; DNA dealkylation

Research Articles on MPG

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Product Notes

The MPG mpg (Catalog #AAA4150376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MPG Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MPG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500 - 1:2000 IHC: 1:50- 1:200 IF: 1:50- 1:100 IP: 1:50- 1:100. Researchers should empirically determine the suitability of the MPG mpg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MPG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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