Growth hormone antagonists Active Protein | G119R active protein
Rat growth hormone antagonists (G119R)
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC.(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 300 nM NaCl, pH 8.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Synonyms
Growth hormone antagonists; Rat growth hormone antagonists (G119R); Rat growth hormone antagonist (G119R); G119R active protein
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 300 nM NaCl, pH 8.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 300 nM NaCl, pH 8.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Form/Format
Sterile-filtered white lyophilized (freeze-dried) powder.
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Ala-Met.
Solubility
It is recommended to reconstitute the lyophilized rGH G119R in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
Dimers and Aggregates
Less than 2% as determined by gel filtration chromatography
Biological Activity
rGH G119R acts as antagonist using an in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is capable of forming a 1:1 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD)and binds to this ECD with affinity similar the the wild type rGH.
Endotoxin
Less than 0.05 ng/ug (IEU/ug) of rat GH G119R.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.752 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman analysis program of protein sequences.
2. Analysis by RP-HPLC, using a calibrated solution of human GH-22K-pl as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 0.752 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman analysis program of protein sequences.
2. Analysis by RP-HPLC, using a calibrated solution of human GH-22K-pl as a Reference Standard.
Preparation and Storage
Lyophilized rGH G119R although stable at room temperature for at least two weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization oGH can be stored at 4 degree C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Addition of carrier protein should be avoided in in vivo experiments to prevent immunic response, Please prevent freeze-thaw cycles.
Related Product Information for G119R active protein
Recombinant rat GH-22K G119R produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids and having a molecular mass of 22 kDa. It is mutated in single amino acid (G119R) according to similar bovine GH mutein described by Chen et al. (1991), Molecular Endocrinology 5:1845-1852. Rat GH antagonist was purified by proprietary chromatographic techniques.
Product Categories/Family for G119R active protein
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Product Notes
The G119R (Catalog #AAA400126) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pr o-Ala-Met. It is sometimes possible for the material contained within the vial of "Growth hormone antagonists, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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