super leptin antagonist Active Protein | SMLA active protein
Mouse super leptin antagonist (mutant D23/L39A/D40A/F41A)
Purity
The purity of mouse super-active leptin antagonist is greater than 98.0% as determined by:(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Synonyms
super leptin antagonist; Mouse super leptin antagonist (mutant D23/L39A/D40A/F41A); Mouse super-active leptin antagonist (SMLA); SMLA active protein
Host
Escherichia Coli
Purity/Purification
The purity of mouse super-active leptin antagonist is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
The mouse super-active leptin antagonist was lyophilized from a concetrated (1mg/ml) solution with 0.003mM NaHCO3.
Sequence
The sequence of the first five N-terminal amino acids of mouse super-active leptin antagonist was determined and was found to be Ala-Val-Pro-Ile-Gln
Solubility
It is recommended to reconstitute the lyophilized mouse super-active leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.
Protein Content
Protein quantization of mouse super-active leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.200 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified mouse super-active leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.
Endotoxin
The endotoxin content of mouse super-active leptin antagonist is less than 0.02 ng/ug (0.2 EU/ug) of mouse leptin antagonist.
Physical Appearance
White lyophilized (freeze-dried) powder.
Biological Activity
Mouse super-active leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Mouse super-active leptin antagonist also inhibits various leptin effects in several in vitro bioassays. The inhibitory activity of mouse super-active leptin antagonist was increased 14 to 60 fold as measured by various criteria such as binding properties to human leptin binding domain (hLBD) and in vitro and in vivo bioassays as compared to mouse leptin antagonist.
Usage
Mouse super-active leptin antagonist is furnished for laboratory research use only. The product may not be used as drugs, agriculutre or pesticidal products, food additives, local application, or household chemicals. It is for research purposes only- commercial use has been liscensed exclusivley to a third party.
Preparation and Storage
Lyophilized mouse super-active leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -20 degree C.
Upon reconstitution of mouse super-active leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization mouse super-active leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Upon reconstitution of mouse super-active leptin antagonist at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization mouse super-active leptin antagonist can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Related Product Information for SMLA active protein
Recombinant mouse leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, mLEP was mutated, resulting in D23L/L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques.
Product Categories/Family for SMLA active protein
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Product Notes
The SMLA (Catalog #AAA400086) is an Active Protein produced from Escherichia Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids of mouse super-acti ve leptin antagonist was determined and was found to be Ala-Val-Pr o-Ile-Gln. It is sometimes possible for the material contained within the vial of "super leptin antagonist, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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