resistin Active Protein | RETN active protein

Human resistin

Cat. Num. AAA400069

• Gene Names: RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
• Purity: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (c) Gel filtration chromatography under non denaturative conditions.
• Synonyms: resistin; Human resistin; RETN active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (c) Gel filtration chromatography under non denaturative conditions.
• Form/Format: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first seven N-terminal amino acids was determined to be Ala-Ser-Ser-Lys-Thr-Leu-Cys.
• Sequence Length: 108

• Solubility: It is recommended to reconstitute the lyophilized resistin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other buffered aqueous solutions containing carrier proteins.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm using the absorbency value of 1.26 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a standard solution of BSA as a Reference Standard.
• Dimers and Aggregates: The protein is a covalent homodimer and contains less than 2% aggregates.
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of human resistin
• Formulation: Recombinant resistin was lyophilized from a concentrated (1mg/ml) solution containg 0.03% NaHCO3.
• Biological Activity: The biological activity was evidenced by resistin ability to prevent insulin-stimulated uptake of deoxy glucose in several cell lines at 10 ng/ml concentration. It also induces phosphrylation of Akt in several cell lines.
• Preparation and Storage: Lyophilized resistin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution it should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please prevent freeze-thaw cycles.

Related Product Information for RETN active protein

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptide contains 11 cysteine residues) referred to as the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone 3). Human resistin is expressed and purified by chromatographic techniques as a 93 amino acid protein without any tag and havin an additional Ala at its N-terminus. It forms a covalent homodimer linked by S-S bond through Cys 7. Resistin may be an important link between obesity and insulin resistance. Resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. It was suggested that resistin suppressed the ability of insulin to stimulate glucose uptake.
The molecular mass of the purified homodimer is 19576 Da.
Recently it was shown that resistin binds to TLR4 in hypothalamus.

Product Categories/Family for RETN active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 9966777
• NCBI GeneID: 56729
• NCBI Accession #: NP_065148.1
• NCBI GenBank Nucleotide #: NM_020415.3
• UniProt Accession #: Q9HD89; Q540D9; Q76B53; D6W649
• Molecular Weight: 8,686 Da
• NCBI Official Full Name: resistin
• NCBI Official Synonym Full Names: resistin
• NCBI Official Symbol: RETN
• NCBI Official Synonym Symbols: ADSF; RSTN; XCP1; FIZZ3; RETN1
• NCBI Protein Information: resistin; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; adipose tissue-specific secretory factor; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; cysteine-rich secreted protein A12-alpha-like 2; cysteine-rich secreted protein FIZZ3; found in inflammatory zone 3; resistin delta2
• UniProt Protein Name: Resistin
• Protein Family: Resistin
• UniProt Gene Name: RETN
• UniProt Synonym Gene Names: FIZZ3; HXCP1; RSTN; ADSF
• UniProt Entry Name: RETN_HUMAN

NCBI Description

This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; nucleus

Molecular Function: hormone activity

Biological Process: fat cell differentiation; response to mechanical stimulus; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; positive regulation of synaptic transmission; response to insulin stimulus; aging

Disease: Diabetes Mellitus, Noninsulin-dependent