growth hormone soluble receptor Active Protein | GHR active protein

Rabbit growth hormone soluble receptor (rbGH binding protein)

Cat. Num. AAA400016

• Gene Names: GHR; GHBP; GHIP
• Purity: Greater than 98.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Synonyms: growth hormone soluble receptor; Rabbit growth hormone soluble receptor (rbGH binding protein); Rabbit growth hormone soluble receptor (so called GH binding protein); GHR active protein

• Host: Escherichia Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Form/Format: White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser.
• Sequence Length: 638

• Solubility: It is recommended to reconstitute the lyophilized rbGHBP in sterile 0.4% NaHCO3 adjusted to pH 10, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Dimers and Aggregates: The purified rbGHBP (28K) consists of > 98% monomers as determined by gel-filtration chromatography.
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of rbGHBP (28 K).
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
• Biological Activity: hGHBP is fully biologically active as evidenced by its ability of forming 2:1 complex with non-primate GHs.
• Preparation and Storage: Lyophilized rbGHBP although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 rbGHBP mg/ml and filter sterilization hGHBP can be stored at 4 degree C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Related Product Information for GHR active protein

Recombinant rbGHBP, one polypeptide chain containing 248 amino acids and having a molecular mass of ~ 28 kDa, Rabbit GHBP was purified by proprietary chromatographic techniques, (see Sakal et al. Prep Biochem Biotechnol. 30:107-23, 2000).

Product Categories/Family for GHR active protein

Soluble receptors

NCBI and Uniprot Product Information

• NCBI GI #: 121180
• NCBI GeneID: 2690
• UniProt Accession #: P10912; Q9HCX2
• Molecular Weight: 69,237 Da
• NCBI Official Full Name: Growth hormone receptor
• NCBI Official Synonym Full Names: growth hormone receptor
• NCBI Official Symbol: GHR
• NCBI Official Synonym Symbols: GHBP; GHIP
• NCBI Protein Information: growth hormone receptor; GH receptor; growth hormone binding protein; serum binding protein; somatotropin receptor
• UniProt Protein Name: Growth hormone receptor
• Protein Family: Growth hormone receptor
• UniProt Gene Name: GHR
• UniProt Synonym Gene Names: GH receptor; GH-binding protein; GHBP
• UniProt Entry Name: GHR_HUMAN

NCBI Description

This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

GH receptor: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Defects in GHR are a cause of Laron syndrome (LARS). A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA). Short stature is defined by a subnormal rate of growth. Belongs to the type I cytokine receptor family. Type 1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p13-p12

Cellular Component: extracellular space; cell surface; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein homodimerization activity; growth factor binding; peptide hormone binding; protein kinase binding

Biological Process: succinate metabolic process; oxaloacetate metabolic process; activation of MAPK activity; positive regulation of multicellular organism growth; tyrosine phosphorylation of JAK2 protein; fatty acid metabolic process; response to estradiol stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; 2-oxoglutarate metabolic process; allantoin metabolic process; receptor internalization; creatinine metabolic process; isoleucine metabolic process; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; valine metabolic process; citrate metabolic process; endocytosis; JAK-STAT cascade; creatine metabolic process; multicellular organismal metabolic process; cellular response to hormone stimulus; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; insulin-like growth factor receptor signaling pathway; response to cycloheximide; taurine metabolic process

Disease: Hypercholesterolemia, Familial; Laron Syndrome