Rabbit anti-Mouse Fibronectin Antibody | anti-Fn1 antibody

Fibronectin, mouse

Cat. Num. AAA397211

• Gene Names: Fn1; Fn; Fn-1; E330027I09
• Reactivity: Mouse
• Applications: Immunofluorescence, Immunohistochemistry, ELISA, immunoassay, Radioimmunoassay
• Purity: Affinity Purified
• Synonyms: Fibronectin; mouse; anti-Fn1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Purity/Purification: Affinity Purified
• Form/Format: Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2477

• Applicable Applications for anti-Fn1 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Radioimmunoassay (RIA)
• Application Notes: IFA: 1:80; IHC-P: 1:1000; ELISA: 1:200
• Immunogen: Purified fibronectin from murine plasma
• Reconstitution: Reconstitute with 0.5 ml of sterile water and store aliquots at -20 degree C.
• Positive Control: Mouse skin
• Incubation Time: IHC(P) 60 min at RT or 2-8 degree C over night
• Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Preparation and Storage: Store at -20 degree C.

Related Product Information for anti-Fn1 antibody

This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Murine fibronectin 100%, murine collagen type I, III and IV <0.1%, murine laminin <0.1% (RIA at 1:10000 dilution).

References

1. Andujar MB, Magloire H, Hartmann DJ, Ville G, Grimaud JA (1985) Early mouse molar root development: cellular changes and distribution of fibronectin, laminin and type-IV collagen. Differentiation. 30(2):111-22. 2.Savino W, Itoh T, Imhof BA, Dardenne M (1986) Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines. Thymus. 8(4):245-56. 3. Villa-Verde DM1, Lagrota-Candido JM, Vannier-Santos MA, Chammas R, Brentani RR, Savino W (1994) Extracellular matrix components of the mouse thymus microenvironment. IV. Modulation of thymic nurse cells by extracellular matrix ligands and receptors. Eur J Immunol. 24(3):659-64. 4. Pankov R and Yamada KM (2002) Fibronectin at a glance. J Cell Sci. Oct 15;115(Pt 20):3861-3. 5. Labat-Robert J (2002) Fibronectin in malignancy. Semin Cancer Biol. Jun;12(3):187-95.

NCBI and Uniprot Product Information

• NCBI GI #: 46849812
• NCBI GeneID: 14268
• NCBI Accession #: NP_034363.1
• NCBI GenBank Nucleotide #: NM_010233.2
• UniProt Accession #: P11276; Q61567; Q61568; Q61569; Q64233; Q80UI4; G5E8B8
• Molecular Weight: 272,538 Da
• NCBI Official Full Name: fibronectin isoform a
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: Fn1
• NCBI Official Synonym Symbols: Fn; Fn-1; E330027I09
• NCBI Protein Information: fibronectin
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: Fn1
• UniProt Synonym Gene Names: FN

Uniprot Description

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.