Rabbit anti-Mouse Collagen Type IV Antibody | anti-Col4a1 antibody

Collagen Type IV, mouse

Cat. Num. AAA397135

• Gene Names: Col4a1; Bru; Raw; Svc; Col4a-1
• Reactivity: Mouse
• Applications: Immunohistochemistry, Immunofluorescence, ELISA, immunoassay, Radioimmunoassay
• Purity: Affinity Purified
• Synonyms: Collagen Type IV; mouse; anti-Col4a1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Purity/Purification: Affinity Purified
• Form/Format: Lyophilized from phosphate buffered solution; no BSA and preservative added.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1669

• Applicable Applications for anti-Col4a1 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA)
• Application Notes: IFA: 1:80; IHC-P: 1:500; ELISA: 1:200
• Immunogen: Purified collagen type IV from murine tumour tissue
• Positive Control: Mouse skin or liver
• Incubation Time: IHC(P) 60 min at RT or 2-8 degree C over night
• Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Preparation and Storage: Store at -20 degree C.

Related Product Information for anti-Col4a1 antibody

Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Murine collagen type IV 100%; murine collagen type I, II and III <0.1%; human collagen type IV and V <0.1%; murine fibronectin <0.1%; murine laminin <0.1% in RIA at 1:5000 dilution.

References

1. Heidet L., Borza D.B., Jouin M., Sich M., Mattei M.G., Sado Y., Hudson B.G., Hastie N., Antignac C., Gubler M.C. (2003) A human-mouse chimera of the alpha3 alpha4 alpha 5 (IV) collagen promoter rescues the renal phenotype in Col4a3-/-Alport mice. Am J Pat. 2. Guerret S., Govignon E., Hartmann D.J., Ronfard V. (2003) Long term remodeling of a bilayered living human skin equivalent (Apligraf) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 3. Mauger A., Emonard H., Hartmann D.J., Foidart J.M., Sengel P. (1987) Immunofluorescent localization of collagen types I, III and IV, fibronectin, laminin, and basement membrane proteoglycan in developing mouse skin. Roux's Arch. Dev. Biol. 196, 295-302.

NCBI and Uniprot Product Information

• NCBI GI #: 161484654
• NCBI GeneID: 12826
• NCBI Accession #: NP_034061.2
• NCBI GenBank Nucleotide #: NM_009931.2
• UniProt Accession #: P02463; Q3UHJ4; Q3UJE7; Q3UQV2; Q53X35; Q6GQS7; Q6PHB5; Q99LQ8
• Molecular Weight: 160,679 Da
• NCBI Official Full Name: collagen alpha-1(IV) chain
• NCBI Official Synonym Full Names: collagen, type IV, alpha 1
• NCBI Official Symbol: Col4a1
• NCBI Official Synonym Symbols: Bru; Raw; Svc; Col4a-1
• NCBI Protein Information: collagen alpha-1(IV) chain
• UniProt Protein Name: Collagen alpha-1(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: Col4a1

NCBI Description

This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.