Rabbit anti-Chicken Collagen Type II Antibody | anti-COL2A1 antibody
Collagen Type II, chicken
Reactivity
Chicken
Applications
Immunohistochemistry, Immunofluorescence, ELISA, immunoassay, Radioimmunoassay
Purity
Affinity Purified
Synonyms
Collagen Type II; chicken; anti-COL2A1 antibody
Host
Rabbit
Reactivity
Chicken
Purity/Purification
Affinity Purified
Form/Format
Lyophilized from phosphate buffered solution; no BSA and preservative added.
Concentration
1 mg/ml (varies by lot)
Sequence Length
859
Applicable Applications for anti-COL2A1 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA)
Application Notes
IF: 1:40
IHC-P: 1:200-1:600
IHC-P: 1:200-1:600
Immunogen
Purified collagen type II from fetal chicken cartilage
Positive Control
Chicken cartilage
Incubation Time
IHC(P) 60 min at RT or 2-8 degree C over night
Pre-Treatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Preparation and Storage
Store at -20 degree C.
Related Product Information for anti-COL2A1 antibody
Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type II 100%, no crossreactivity with chicken collagen type I and III, no crossreactivity with human, mouse and rat collagen type II.
References
1. Tacchetti C., Quarto R., Nitsch L., Hartmann D.J., Cancedda R. (1987) In vitro morphogenesis of chick embryo hypertrophic cartilage. J. Cell. Biol. 105, 999-1006. 2. Mallein-Gerin F., Ruggiero F., Garrone R. (1990) Proteoglycan core protein and type II collagen gene expressions are not correlated with cell shape changes during low density chondrocyte cultures. Differentiation 43, 204-211. 3. Anselme K., Lanel B., Gentil C., Hardouin P., Marie P.J., Sigot-Luizard M.F. (1994) Bone organotypic culture method: a model for cytocompatibility testing of biomaterials. Cells and Materials 4, 113-123. 4. Rousseau J.C., Farjanel J., Boutillon M.M., Hartmann D.J., van der Rest M., Moradi-Ameli M. (1996) Processing of Type XI Collagen. J. Biol. Chem. 271, 23743-23748.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
74,257 Da
NCBI Official Full Name
Collagen alpha-1(II) chain
UniProt Protein Name
Collagen alpha-1(II) chain
UniProt Gene Name
COL2A1
Uniprot Description
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
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Product Notes
The Collagen Type II col2a1 (Catalog #AAA397124) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen Type II, chicken reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen Type II can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA). IF: 1:40 IHC-P: 1:200-1:600. Researchers should empirically determine the suitability of the Collagen Type II col2a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen Type II, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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