Mouse anti-Human Cytokeratin 14 Antibody | anti-KRT14 antibody
Cytokeratin 14
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
Cytokeratin 14; anti-KRT14 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG3
Clone Number
LL002
Form/Format
Solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.
Sequence Length
471
Applicable Applications for anti-KRT14 antibody
Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes
Working Concentration: (RTU) neat
Immunogen
C-terminal 15-AA peptide of cytokeratin 14 conjugated to thyroglobulin
Positive Control
Bronchial epithelium
Incubation Time
60 min at RT
Pre-Treatment
Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid G ()
Pre-Treatment
Heat pre-treatment of formalin-fixed tissue with Unmasking Fluid G ()
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for anti-KRT14 antibody
Cytokeratin 14 (CK14) belongs to the acid subfamily of CKs (Type I) and is characteristic of stratifying epithelia (trachea, bronchi, cervix, epidermis, sweat glands). The antibody to CK14 is helpful to distinguish between stratifying epithelia and simple epithelia, which don't express this type of cytokeratins. Tumours of squamous cell origin will be stained with this antibody. Cytokeratins are a group of water-insoluble filamentous proteins. They are constituents of the cytoskeleton of epidermal cells and other epithelial cells. Up to now 20 different cytokeratins have been characterised by gel electrophoresis. They have been subdivided into a basic and an acid subfamily. Most frequently the nomenclature according to Moll et al. (1982) is used. Human cytokeratin 14.
References
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff. 2. Sun T.-T. Tseng S.C.G., Huang A.J.W., Cooper D., Lynch M.H., Weiss R., Eichner R., and Schermer (1985) Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments, Wang E. et al. eds. N.Y. Acad. Sci. 455, pp 307 ff. 3. Harnden P. and Southgate J. (1997) Cytokeratin 14 as a marker of squamous differentiation in transitional cell carcinomas. J Clin Pathol. 50(12):1032-1033. 4. Sarbia M, Verreet P, Bittinger F, Dutkowski P, Heep H, Willers R, Gabbert HE. (1997) Basaloid squamous cell carcinoma of the esophagus: diagnosis and prognosis. Cancer 79(10):1871-1878. 5. Lakhani S.R., O'Hare M.J., Monaghan P., Winehouse J., Gazet J.C., Sloane J.P. (1995) Malignant myoepithelioma (myoepithelial carcinoma) of the breast: a detailed cytokeratin study. J. Clin. Pathol. 48(2):164-167.
NCBI and Uniprot Product Information
Uniprot Description
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
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Product Notes
The Cytokeratin 14 krt14 (Catalog #AAA397094) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 14 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 14 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin/Paraffin. Working Concentration: (RTU) neat. Researchers should empirically determine the suitability of the Cytokeratin 14 krt14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 14, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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