Rabbit anti-Human Rit Polyclonal Antibody | anti-Rit antibody

Rit

Cat. Num. AAA395748

• Gene Names: RIT1; RIT; RIBB; ROC1
• Reactivity: Human
• Applications: Western Blot
• Purity: Ammonium Sulfate Precipitation
• Synonyms: Rit; Polyclonal Antibody; anti-Rit antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 183

• Applicable Applications for anti-Rit antibody: Western Blot (WB)
• Immunogen: His-tagged recombinant human Rit protein
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-Rit antibody

Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64% amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100% conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Product Categories/Family for anti-Rit antibody

Polyclonal Antibody; G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 378744212
• NCBI GeneID: 6016
• NCBI Accession #: NP_001243749.1
• NCBI GenBank Nucleotide #: NM_001256820.1
• UniProt Accession #: Q92963; O00646; O00720; Q5VY89; Q5VY90; B4DQE8
• Molecular Weight: 35
• NCBI Official Full Name: GTP-binding protein Rit1 isoform 3
• NCBI Official Synonym Full Names: Ras-like without CAAX 1
• NCBI Official Symbol: RIT1
• NCBI Official Synonym Symbols: RIT; RIBB; ROC1
• NCBI Protein Information: GTP-binding protein Rit1; GTP-binding protein Roc1; ras-like without CAAX protein 1; Ric-like, expressed in many tissues; ras-like protein expressed in many tissues
• UniProt Protein Name: GTP-binding protein Rit1
• UniProt Gene Name: RIT1
• UniProt Synonym Gene Names: RIBB; RIT; ROC1
• UniProt Entry Name: RIT1_HUMAN

NCBI Description

This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

Function: Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. Ref.11

Enzyme regulation: Alternate between an inactive form bound to GDP and an active form bound to GTP.

Subunit structure: Interacts with MLLT4, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Strongly interacts with BRAF, but only weakly with RAF1. BARF and RAF1 association is dependent upon the GTP-bound state. Interacts with RGL3

By similarity. Ref.4 Ref.11

Subcellular location: Cell membrane.

Tissue specificity: Expressed in many tissues.

Miscellaneous: Stimulation of the NGF and EGF receptor signaling pathways results in rapid and prolonged activation.Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members.

Sequence similarities: Belongs to the small GTPase superfamily. Ras family.