Mouse anti-Human Calpain I, II (small subunit) agarose Monoclonal Antibody | anti-CAPN2 antibody

Calpain I, II (small subunit) agarose

Cat. Num. AAA395124

• Gene Names: CAPN2; CANP2; mCANP; CANPL2; CANPml
• Reactivity: Human
• Applications: Immunoprecipitation
• Purity: Protein A/G Chromatography
• Synonyms: Calpain I; II (small subunit) agarose; Monoclonal Antibody; Mouse anti calpain I; II small subunit; p30; agarose conjugate; anti-CAPN2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 28F3
• Purity/Purification: Protein A/G Chromatography
• Form/Format: Provided as a concentration of 1 mg/ml in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, 3mM sodium azide.
• Sequence Length: 622

• Applicable Applications for anti-CAPN2 antibody: Immunoprecipitation (IP)
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-CAPN2 antibody

The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, -calpain is active at micromolar (M) concentrations of Ca ++. In addition to the ubiquitously expressed m- and calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Product Categories/Family for anti-CAPN2 antibody

Monoclonal Antibody; Structural Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 225703100
• NCBI GeneID: 824
• NCBI Accession #: NP_001139540.1
• NCBI GenBank Nucleotide #: NM_001146068.1
• UniProt Accession #: P17655; Q16738; Q6PJT3; Q8WU26; Q9HBB1; A6NDG7; B7ZA96; E7ES58
• Molecular Weight: 30
• NCBI Official Full Name: calpain-2 catalytic subunit isoform 2
• NCBI Official Synonym Full Names: calpain 2, (m/II) large subunit
• NCBI Official Symbol: CAPN2
• NCBI Official Synonym Symbols: CANP2; mCANP; CANPL2; CANPml
• NCBI Protein Information: calpain-2 catalytic subunit; CANP 2; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain, large polypeptide L2; calpain 2, large [catalytic] subunit; calcium-activated neutral proteinase 2
• UniProt Protein Name: Calpain-2 catalytic subunit
• Protein Family: Calpain
• UniProt Gene Name: CAPN2
• UniProt Synonym Gene Names: CANPL2; CANP 2; M-calpain
• UniProt Entry Name: CAN2_HUMAN

NCBI Description

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

Function: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Catalytic activity: Broad endopeptidase specificity.

Cofactor: Binds 7 calcium ions

By similarity.

Enzyme regulation: Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Subunit structure: Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Subcellular location: Cytoplasm. Cell membrane. Note: Translocates to the plasma membrane upon Ca2+ binding.

Tissue specificity: Ubiquitous.

Sequence similarities: Belongs to the peptidase C2 family.Contains 1 calpain catalytic domain.Contains 3 EF-hand domains.

Sequence caution: The sequence AAH07686.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.The sequence AAH11828.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.