Mouse anti-Human, Mouse Fibronectin Monoclonal Antibody | anti-Fn1 antibody

Fibronectin

Cat. Num. AAA395082

• Gene Names: Fn1; Fn; Fn-1; E330027I09
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: Protein A/G Chromatography
• Synonyms: Fibronectin; Monoclonal Antibody; anti-Fn1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: EP5
• Purity/Purification: Protein A/G Chromatography
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 2477

• Applicable Applications for anti-Fn1 antibody: Immunohistochemistry (IHC) Frozen/Paraffin
• Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with T-cell lymphoma biopsy and mouse myeloma cells.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-Fn1 antibody

Fibronectins are a family of plasma and extracellular matrix glycoproteins that contain multiple sites for various ligands and cells. The region of fibronectin that mediates cell adhesion in a majority of cell types is in the carboxy terminal domain and involves the binding of the Arg-Gly-Asp (RGD) sequence to a specific polypeptide cell surface receptor. Although the mechanisms of fibronectin extracellular matrix assembly are not well understood, specific antibodies have been used to determine the domains involved in matrix assembly.

Product Categories/Family for anti-Fn1 antibody

Monoclonal Antibody; Extracellular Matrix

NCBI and Uniprot Product Information

• NCBI GI #: 46849812
• NCBI GeneID: 14268
• NCBI Accession #: NP_034363.1
• NCBI GenBank Nucleotide #: NM_010233.2
• UniProt Accession #: P11276; Q61567; Q61568; Q61569; Q64233; Q80UI4; G5E8B8
• Molecular Weight: 272,538 Da
• NCBI Official Full Name: fibronectin isoform a
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: Fn1
• NCBI Official Synonym Symbols: Fn; Fn-1; E330027I09
• NCBI Protein Information: fibronectin
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: Fn1
• UniProt Synonym Gene Names: FN
• UniProt Entry Name: FINC_MOUSE

Uniprot Description

Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling

By similarity.

Subunit structure: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, LGALS3BP and COL13A1 and COMP

By similarity. Interacts with TNR; interaction mediates inhibition of cell adhesion and neurite outgrowth. Interacts with FBLN7. Interacts with FST3

By similarity. Ref.13

Subcellular location: Secreted › extracellular space › extracellular matrix.

Tissue specificity: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.

Induction: Glucocorticoids suppressed mRNA expression and protein synthesis. Ref.11

Post-translational modification: Sulfated

By similarity.Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).Phosphorylation sites are present in the extracellular medium

By similarity.Proteolytic processing produces the C-terminal NC1 peptide, anastellin

By similarity.

Sequence similarities: Contains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 17 fibronectin type-III domains.