SDS-Page
(Recombinant KAT5 protein gel. 10% SDS-PAGE Coomassie staining. MW: 63.1 kDa Purity: >90%)
KAT5 recombinant protein
Recombinant KAT5 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant KAT5 protein gel. 10% SDS-PAGE Coomassie staining. MW: 63.1 kDa Purity: >90%)
SDS-Page
(Recombinant KAT5 protein gel. 10% SDS-PAGE Coomassie staining. MW: 63.1 kDa Purity: >90%)
Western Blot (WB)
(Western blot for Recombinant KAT5 protein activity 0.5 ug Histone H4 was incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody . Western blot was used to detect the production of acetylated histone H4.)
Western Blot (WB)
(Western blot for Recombinant KAT5 protein activity 0.5 ug Histone H4 was incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody . Western blot was used to detect the production of acetylated histone H4.)
Western Blot (WB)
(Western blot for Recombinant KAT5 protein activity 2 ?g Recombinant Mononucleosomes H3.1 were incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody and H3 antibody . Western blot was used to detect the production of acetylated histone H4. H3 was detected as a loading control.)
Western Blot (WB)
(Western blot for Recombinant KAT5 protein activity 2 ?g Recombinant Mononucleosomes H3.1 were incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody and H3 antibody . Western blot was used to detect the production of acetylated histone H4. H3 was detected as a loading control.)
Background: KAT5 (Lysine Acetyltransferase 5), also called as TIP60, is a member of the MYST family of histone acetyl transferases. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. NuA4 histone acetyltransferase complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. KAT5 is also a component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. Besides, it also acetylates non-histone proteins, such as ATM, NR1D2, RAN, FOXP3, ULK1 and RUBCNL/Pacer.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Research Articles on KAT5
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Product Notes
The KAT5 (Catalog #AAA389324) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KAT5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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