p300 recombinant protein

Recombinant p300 protein

Cat. Num. AAA389215

• Gene Names: EP300; p300; KAT3B; RSTS2
• Synonyms: p300; Recombinant p300 protein; EP300; KAT3B; RSTS2; p300 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant p300 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant p300 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant p300 protein gel 8% SDS-PAGE Coomassie staining MW: 265.4 kDa Purity: >75% )

Western Blot (WB)

(Recombinant p300 protein activity assay 0.5 ug of Histone H4 were incubated with 0 (-), 12.5 (+), 25 (++), or 50 (+++) ng of p300 protein, respectively in a 20 ul reaction system including 50 mM Tris pH 8.0, 1 mM TCEP, 0.1 mM EDTA, 50 ng/ul BSA and 20 uM acetyl-CoA for 30 minutes at 30 degree C. For each reaction, 7.5 ul product was run on a 12.5% SDS-PAGE gel and detected by Western Blot. p300 only was used as negative control. H4 pan-acetyl antibody was used to recognize products.)

Related Product Information for p300 recombinant protein

Short Description: Recombinant p300 protein was expressed in a baculovirus expression system as full-length protein (accession number NP_001420.2) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 265.4 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: E1A binding protein p300 (EP300) or p300, also known as KAT3B or RSTS2, regulates cellular growth and differentiation and is also important in preventing tumor growth. It binds to transcription factors and functions as a coactivator of transcription. The p300 domain structure that facilitates interaction with transcription factors includes the bromodomain, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/ histidine regions (TAZ1and TAZ2) and the interferon response binding domain (IBiD). Its interaction with adenovirus E1A protein is thought regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for nonhistone targets. Specifically, p300 acetylates 'Lys131' of ALX1 and acts as its coactivator in the presence of CREBBP. p300 is also thought to indirectly increase the transcriptional activity of p53 through acetylation of SIRT2 and subsequent attenuation of its deacetylase function. Additionally, HDAC1 acetylation by p300 leads to HDAC1 inactivation. p300 also acts as a TFAP2A-mediated transcriptional coactivator in the presence of CITED2 and as a coactivator of NEUROD1-dependent transcription of secretin and p21. Additionally, p300 binds to phosphorylated CREB and mediates cAMP gene regulation. It also regulates terminal differentiation of intestinal epithelial cells. In the case of HIV-1 infection, p300 is recruited by the viral protein TAT and regulates TAT's transactivating activity, and may aid induction of chromatin remodeling of proviral genes.

Product Categories/Family for p300 recombinant protein

Recombinant Proteins; Bromodomain

NCBI and Uniprot Product Information

• NCBI GI #: 50345997
• NCBI GeneID: 2033
• NCBI Accession #: NP_001420.2
• NCBI GenBank Nucleotide #: NM_001429.3
• UniProt Accession #: Q09472; B1AKC2
• Molecular Weight: 264,161 Da
• NCBI Official Full Name: histone acetyltransferase p300
• NCBI Official Synonym Full Names: E1A binding protein p300
• NCBI Official Symbol: EP300
• NCBI Official Synonym Symbols: p300; KAT3B; RSTS2
• NCBI Protein Information: histone acetyltransferase p300; p300 HAT; E1A-binding protein, 300kD; E1A-associated protein p300
• UniProt Protein Name: Histone acetyltransferase p300
• UniProt Gene Name: EP300
• UniProt Synonym Gene Names: P300; p300 HAT
• UniProt Entry Name: EP300_HUMAN

NCBI Description

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

p300: a histone acetyltransferase and transcriptional co-activator that regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Related to CPB (CREB-binding protein), and like CPB can stimulate transcription through activation of CREB. Specifically inhibited by the adenovirus oncoprotein E1A. A co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Methylated at R580 and R604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates the apoptotic response. Also methylated at R2142 by CARM1, which impairs interaction with NCoA2.

Protein type: Nuclear receptor co-regulator; EC 2.3.1.48; Acetyltransferase; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; histone acetyltransferase complex

Molecular Function: lysine N-acetyltransferase activity; transcription activator binding; zinc ion binding; p53 binding; chromatin DNA binding; transcription coactivator activity; beta-catenin binding; transcription factor binding; protein binding; histone acetyltransferase activity; DNA binding; androgen receptor binding; acetyltransferase activity; transferase activity, transferring acyl groups; chromatin binding; nuclear hormone receptor binding

Biological Process: circadian rhythm; establishment and/or maintenance of chromatin architecture; positive regulation of protein binding; viral reproduction; regulation of cell cycle; apoptosis; heart development; positive regulation of transcription of target genes involved in unfolded protein response; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of interferon type I production; G2/M transition of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; nervous system development; Notch signaling pathway; skeletal muscle development; somitogenesis; internal peptidyl-lysine acetylation; transcription, DNA-dependent; protein stabilization; organ morphogenesis; protein amino acid acetylation; response to estrogen stimulus; response to hypoxia; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; mitotic cell cycle; internal protein amino acid acetylation; lung development; N-terminal peptidyl-lysine acetylation

Disease: Rubinstein-taybi Syndrome 2; Rubinstein-taybi Syndrome 1; Colorectal Cancer