STAT3 recombinant protein

Recombinant STAT3 protein

Cat. Num. AAA389152

• Gene Names: STAT3; APRF; HIES
• Synonyms: STAT3; Recombinant STAT3 protein; Signal Transducer And Activator Of Transcription 3; APRF; HIES; ADMIO; STAT3 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant STAT3 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant STAT3 protein gel 10% SDS-PAGE Coomassie staining MW: 89.2 kDa Purity: >90% )

Related Product Information for STAT3 recombinant protein

Short Description: Recombinant human STAT3 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_644805.1) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 89.2 kDa.

Background: STAT3 (Signal Transducer And Activator Of Transcription 3), also known as APRF (Acute-Phase Response Factor) or ADMIO, HIES, is a signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, STAT3 can recruit coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. Deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. STAT3 can bind to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. It also acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells. It is involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.

Product Categories/Family for STAT3 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 21618340
• NCBI GeneID: 6774
• NCBI Accession #: NP_644805.1
• NCBI GenBank Nucleotide #: NM_139276.2
• UniProt Accession #: P40763; O14916; Q9BW54; A8K7B8
• Molecular Weight: 88,068 Da
• NCBI Official Full Name: signal transducer and activator of transcription 3 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3 (acute-phase response factor)
• NCBI Official Symbol: STAT3
• NCBI Official Synonym Symbols: APRF; HIES
• NCBI Protein Information: signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT3
• UniProt Synonym Gene Names: APRF
• UniProt Entry Name: STAT3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; ligand-dependent nuclear receptor activity; signal transducer activity; DNA binding; sequence-specific DNA binding; protein kinase binding; transcription factor binding; protein phosphatase binding; transcription factor activity; CCR5 chemokine receptor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; viral reproduction; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; signal transduction; response to estradiol stimulus; negative regulation of cell proliferation; astrocyte differentiation; regulation of transcription, DNA-dependent; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; response to drug; nervous system development; intracellular receptor-mediated signaling pathway; eating behavior; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; cell proliferation; response to ethanol; sexual reproduction; positive regulation of transcription from RNA polymerase II promoter; eye photoreceptor cell differentiation; cell motility; phosphorylation

Disease: Hyper-ige Recurrent Infection Syndrome, Autosomal Dominant; Autoimmune Disease, Multisystem, Infantile-onset