Western Blot (WB)
(RANK mAb tested by Western blot. RANK detection by Western blot. The analysis was performed using 293 cells transfected with RANK cDNA and RANK mAb at a 2 ug/ml dilution. A single protein band of approximate mol. wt. of 97 kDa was detected.)
Mouse anti-Human RANK Monoclonal Antibody | anti-RANK antibody
RANK antibody (mAb)(Clone 9A725)
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Western Blot (WB)
(RANK mAb tested by Western blot. RANK detection by Western blot. The analysis was performed using 293 cells transfected with RANK cDNA and RANK mAb at a 2 ug/ml dilution. A single protein band of approximate mol. wt. of 97 kDa was detected.)
Western Blot (WB)
(RANK mAb tested by Western blot. RANK detection by Western blot. The analysis was performed using 293 cells transfected with RANK cDNA and RANK mAb at a 2 ug/ml dilution. A single protein band of approximate mol. wt. of 97 kDa was detected.)
Background: RANK (TNFRSF11A, CD265, PDB2, TRANCER) can interact with various TRAF family proteins (TRAF2, TRAF5), through which this receptor induces the activation of NFkB and MAPK8/JNK. RANK and its ligand are important regulators of the interaction between T cells and dendritic cells. RANK is also an essential mediator for osteoclast and lymph node development. Studies of the mouse counterpart suggest that this receptor directly mediates the osteoprotegerin (OPG) ligand (OPGL)-induced oteoclastogenesis in osteoclast precursor cells.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012]
Uniprot Description
TNFRSF11A: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 18q22.1
Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane
Molecular Function: cytokine binding; protein binding; receptor activity; transmembrane receptor activity; tumor necrosis factor receptor activity
Biological Process: activation of NF-kappaB transcription factor; adaptive immune response; cell-cell signaling; circadian thermoregulation; inflammatory response; osteoclast differentiation; positive regulation of cell proliferation; positive regulation of JNK activity; positive regulation of transcription factor activity; regulation of apoptosis; response to cytokine stimulus; response to lipopolysaccharide; signal transduction; tumor necrosis factor-mediated signaling pathway
Disease: Familial Expansile Osteolysis; Osteopetrosis, Autosomal Recessive 7; Paget Disease Of Bone
Research Articles on RANK
Similar Products
Product Notes
The RANK tnfrsf11a (Catalog #AAA388678) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RANK antibody (mAb)(Clone 9A725) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RANK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the RANK tnfrsf11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RANK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
